The CD58 (Anti–LFA-3) antibody, clone L306.4, is derived from the hybridization of Sp2/0 mouse myeloma cells with spleen cells isolated from C3H/HeN mice immunized with Lc–LFA-3 mouse L-cell transfectant.
The CD58 antibody (Anti–LFA-3) recognizes a 40- to 65-kilodalton (kDa) cell-surface glycoprotein that is a member of the immunoglobulin superfamily. The CD58 antigen, known as the lymphocyte function-associated antigen-3 (LFA-3), mediates cellular adhesion and participates in signal transduction when it binds to its ligand, the CD2 antigen. Cellular interactions regulated by the CD58/CD2 antigens are involved in the antigen-independent adhesion pathway and cytotoxic T lymphocyte (CTL) activity. The CD58 antigen has two isoforms. One isoform is anchored in the cell membrane by a glycophosphatidyl inositol tail, while the other has a transmembrane hydrophobic segment and a cytoplasmic segment composed of 12 amino acids.
Development References (8)
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