CD1a PE

BD™ CD1a PE

Name: CD1a PE
Brand: BD™
SKU: 341641

Clone SK9

(ASR)

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Product Details

Brand: BD™

Alternative Name: CD1; T6/Leu-6; R4; T6; HTA-1; FCB6

Reactivity: Human

Isotype: Mouse IgG2b, κ

Immunogen: Human Thymocytes

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Entrez Gene ID: 909

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: ASR

Preparation And Storage

Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341641 Rev. 1

Antibody Details

SK9

The CD1a antibody, clone SK9,1 is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from CD-1 mice immunized with human thymocytes.

The CD1a antibody recognizes a 49-kilodalton (kDa) type I transmembrane glycoprotein that associates non-covalently with β₂-microglobulin.

341641 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

341641 Rev.1

Citations & References

Development References (8)

Boumsell L, Amiot M, Raynal B, Gay-Bellile V, Caillou B, Bernard A. Epitope groups of CD1 molecules. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:289-302.
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Dalloul AH, Patry C, Salamero J, Canque B, Grassi F, Schmitt C. Functional and phenotypic analysis of thymic CD34+ CD1a- progenitor-derived dendritic cells: predominance of CD1a- differentiation pathway. J Immunol. 1999; 162:5821-5828. (Biology).
Ito T, Inaba M, Inaba K, et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells.. J Immunol. 1999; 163(3):1409-19. (Biology). View Reference
Martin LH, Calabi F, Lefebvre FA, Bilsland CA, Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.. Proc Natl Acad Sci USA. 1987; 84(24):9189-93. (Biology). View Reference
Wood GS, Burns BF, Dorfman RF, Warnke RA. The immunohistology of non-T cells in the acquired immunodeficiency syndrome.. Am J Pathol. 1985; 120(3):371-9. (Biology). View Reference
Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). View Reference

View All (8)

341641 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Refer to manufacturer's instructions for use and related User Manuals and Technical Data Sheets before using this product as described.

Comparisons, where applicable, are made against older BD technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.