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Purified Mouse anti-PDGFRβ (CD140b) (pY771)
Purified Mouse anti-PDGFRβ (CD140b) (pY771)

Western blot analysis of PDGFRβ (pY771).  Lysates from control (left panel) and PDGF-treated (right panel) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRβ (CD140b) (pY771) at concentrations of 0.016 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6).  PDGFRβ (pY771) is identified as a band of 180 kDa in the treated cells.

Purified Mouse anti-PDGFRβ (CD140b) (pY771)

PDGFRβ (pY771) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-PDGFRβ (CD140b) (pY771) with Hematoxylin counterstaining.  Original magnification: 20X.

Western blot analysis of PDGFRβ (pY771).  Lysates from control (left panel) and PDGF-treated (right panel) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRβ (CD140b) (pY771) at concentrations of 0.016 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6).  PDGFRβ (pY771) is identified as a band of 180 kDa in the treated cells.

PDGFRβ (pY771) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-PDGFRβ (CD140b) (pY771) with Hematoxylin counterstaining.  Original magnification: 20X.

Product Details
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BD Pharmingen™
CD140b (pY771)
Mouse (QC Testing), Human (Tested in Development)
Mouse BALB/c IgG2a, κ
Phosphorylated Human PDGFRβ Peptide
Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
180 kDa
0.5 mg/ml
AB_647267
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558361 Rev. 2
Antibody Details
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J23-618

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase.  PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides.  Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs.  Ligand binding induces dimerization and activation of the receptor.  Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated.  PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ.

The J23-618 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b.  pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.

558361 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558361 Rev.2
Citations & References
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Development References (3)

  1. Claesson-Welsh L. Platelet-derived growth factor receptor signals. J Biol Chem. 1994; 269(51):32023-32026. (Biology).
  2. Ekman S, Kallin A, Engstrom U, Heldin CH, Ronnstrand L. SHP-2 is involved in heterdimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor. Oncogene. 2002; 21(12):1870-1875. (Biology).
  3. Liu J, Oh P, Horner T, Rogers RA, Schnitzer JE. Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains. J Biol Chem. 1997; 272(11):7211-7222. (Biology). View Reference
558361 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.