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Purified Mouse Anti-Cytokeratin 5/8
Purified Mouse Anti-Cytokeratin 5/8

Western blot analysis of Cytokeratin 5/8. Lysate from HeLa cells was probed with anti-cytokeratin 5/8 (clone RCK102, Cat. No. 550505) at concentrations of 1 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). Cytokeratin 5/8 is identified as a doublet of ~54/52 kDa.

Western blot analysis of Cytokeratin 5/8. Lysate from HeLa cells was probed with anti-cytokeratin 5/8 (clone RCK102, Cat. No. 550505) at concentrations of 1 (lane 1), 0.5 (lane 2), and 0.25 µg/ml (lane 3). Cytokeratin 5/8 is identified as a doublet of ~54/52 kDa.

Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat, Hamster, Pig, Dog, Rabbit (Reported)
Mouse IgG1
Human lung cancer cell line (MR21)
Western blot (Routinely Tested), Immunohistochemistry (Reported)
54/52 kDa
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (0.5 - 1 µg/ml). HeLa cell lysate (Cat. No. 611449) is recommended as a positive control. Additional reported applications include immunohistochemistry on methanol fixed tissue, but this is application is not tested at BD Pharmingen.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550505 Rev. 1
Antibody Details
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Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Type I and II IF proteins are designated keratins based upon whether they are acidic (I) or basic (II) and are characterized by their remarkable biochemical diversity. A subgroup of keratins, designated cytokeratins, are represented in epithelial tissues by at least 20 different polypeptides, designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an  epithelium. Cytokeratin 5 has a molecular weight of approximately 58 kDa (NCBI Accession number P13647) while cytokeratin 8 is approximately 53 kDa (NCBI Accession number P05787). We observe cytokeratin 5 to migrate at approximately 54 kDa and cytokeratin 8 at approximately 52 kDa.

The antibody recognizes cytokeratins 5 and 8 from human, mouse, rat, hamster, rabbit, canine, and swine. The antibody stains all epithelial tissue; it does not stain non-epithelial tissue. The human lung cancer cell line (MR21) was used as the immunogen.

550505 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550505 Rev.1
Citations & References
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Development References (5)

  1. Broers JL, Carney DN, Klein Rot M, et al . Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986; 83:37-60. (Immunogen). View Reference
  2. Broers JL, Ramaekers FC, Rot MK, et al. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res. 1988; 48(11):3221-3229. (Biology). View Reference
  3. Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000:795-847.
  4. Ramaekers F, Huysmans A, Schaart G, Moesker O, Vooijs P. Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res. 1987; 170(1):235-249. (Clone-specific: Western blot). View Reference
  5. Smedts F, Ramaekers F, Robben H, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990; 136(3):657-668. (Clone-specific: Immunohistochemistry). View Reference
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550505 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.