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Purified Mouse Anti-NHE
Purified Mouse Anti-NHE

Western blot analysis for NHE on a HEK-293 cell lysate (Human embryonic kidney cells; ATCC CRL-1573).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-NHE antibody.

Purified Mouse Anti-NHE

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis for NHE on a HEK-293 cell lysate (Human embryonic kidney cells; ATCC CRL-1573).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-NHE antibody.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Na+/H+ Exchangers
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Rat NHE-1 aa. 682-801
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
92 kDa
250 µg/ml
AB_399261
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
611775 Rev. 1
Antibody Details
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54/NHE

The extrusion of H+ in exchange for extracellular Na+ is important for many cellular processes, such as pH homeostasis, volume regulation, and transepithelial ion and water transport. Na+/H+ Exchangers (NHE) are integral membrane proteins that mediate electroneutral exchange of one Na+ ion for one H+ ion. Six NHE forms, NHE-1 thru -6, have been identified. NHE-1 and NHE-6 are widely expressed, while the other NHE forms have restricted expression. The common structure of all NHE forms includes 10-12 N-terminal membrane (M) spanning regions, a conserved M6 and M7 region that may participate in ion transport, and a large C-terminal cytoplasmic region that may be involved in the regulation of ion exchange activity. NHE-1, for example, contains 12 M regions plus domains for volume sensitivity, calmodulin-binding, CHP-binding, and PKC phosphorylation in the cytoplasmic region. Regulation of NHE-1 ion exchange activity may occur through phosphoinositide binding, as well as PKC- and PKA-dependent signaling pathways. Mutation of NHE-1 in mice causes neuronal death in the cerebellum and brainstem, leading to ataxia and seizures. Thus, NHE-1 is a ubiquitous NHE that is essential for normal brain function.

Although this antibody was developed against the NHE-1 antigen, investigators should note that crossreactivity to other NHE isoforms or variants may be possible.

611775 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611775 Rev.1
Citations & References
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Development References (5)

  1. Aharonovitz O, Zaun HC, Balla T, York JD, Orlowski J, Grinstein S. Intracellular pH regulation by Na(+)/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate. J Cell Biol. 2000; 150(1):213-224. (Biology). View Reference
  2. Cox GA, Lutz CM, Yang CL. Sodium/hydrogen exchanger gene defect in slow-wave epilepsy mutant mice. Cell. 1997; 91(1):139-148. (Biology). View Reference
  3. DNA Damage-Induced Bcl-XL Deamidation Is Mediated by NHE-1 Antiport Regulated Intracellular pH. DNA Damage-Induced Bcl-XL Deamidation Is Mediated by NHE-1 Antiport Regulated Intracellular pH. 2007; 5(1)Available: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1702560/pdf/pbio.0050001.pdf JAN 2007.
  4. Kandasamy RA, Yu FH, Harris R, Boucher A, Hanrahan JW, Orlowski J. Plasma membrane Na+/H+ exchanger isoforms (NHE-1, -2, and -3) are differentially responsive to second messenger agonists of the protein kinase A and C pathways. J Biol Chem. 1995; 270(49):29209-29216. (Biology). View Reference
  5. Orlowski J, Kandasamy RA, Shull GE. Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins. J Biol Chem. 1992; 267(13):9331-9339. (Biology). View Reference
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611775 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.