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Purified Mouse Anti-Moesin
Purified Mouse Anti-Moesin

Western blot analysis of Moesin on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Moesin antibody.

Purified Mouse Anti-Moesin

Immunofluorescence staining of A498 cells (Human kidney carcinoma; ATCC HTB-44).

Western blot analysis of Moesin on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Moesin antibody.

Immunofluorescence staining of A498 cells (Human kidney carcinoma; ATCC HTB-44).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Moesin aa. 554-564
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
78 kDa
250 µg/ml
AB_397785
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610401 Rev. 1
Antibody Details
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38/Moesin

Moesin belongs to a family of proteins that includes ezrin and radixin. These proteins are co-expressed in a variety of cells. They localize to the cytoskeleton and modify interactions between cytoskeletal and membrane proteins. There is approximately 70% homology between moesin and ezrin and 80% homology between moesin and radixin. Moesin is intracellular and contains multiple conserved domains that are putative phosphorylation sites. It has a calculated molecular weight of 67.8 kDa, but migrates in SDS-PAGE at approximately 78 kDa. This discrepancy is likely due to a charged alpha-helical region within the protein.

610401 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610401 Rev.1
Citations & References
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Development References (5)

  1. Lankes WT, Furthmayr H. Moesin: a member of the protein 4.1-talin-ezrin family of proteins. Proc Natl Acad Sci U S A. 1991; 88(19):8297-8301. (Biology). View Reference
  2. Lankes WT, Schwartz-Albiez R, Furthmayr H. Cloning and sequencing of porcine moesin and radixin cDNA and identification of highly conserved domains. Biochim Biophys Acta. 1993; 1216(3):479-482. (Biology). View Reference
  3. Parlato S, Giammarioli AM, Logozzi M, et al. CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway. EMBO J. 2000; 19(19):5123-5134. (Biology: Immunofluorescence, Western blot). View Reference
  4. Poliak S, Matlis S, Ullmer C, Scherer SS, Peles E. Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells. J Cell Biol. 2002; 159(2):361-371. (Biology: Immunofluorescence). View Reference
  5. Seveau S, Keller H, Maxfield FR, Piller F, Halbwachs-Mecarelli L. Neutrophil polarity and locomotion are associated with surface redistribution of leukosialin (CD43), an antiadhesive membrane molecule. Blood. 2000; 95(8):2462-2470. (Biology: Immunofluorescence, Western blot). View Reference
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610401 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.