Skip to main content Skip to navigation
Purified Mouse Anti-Human TopBP1
Purified Mouse Anti-Human TopBP1

Western blot analysis of TopBP1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human TopBP1 antibody.

Purified Mouse Anti-Human TopBP1

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of TopBP1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human TopBP1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Topoisomerase II Binding Protein 1
Human (QC Testing)
Mouse IgG1
Human TopBP1 aa. 204-416
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
161 kDa
250 µg/ml
AB_399355
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611874 Rev. 1
Antibody Details
Down Arrow Up Arrow
33/TopBP1

Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIα and topo IIβ. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. DNA Topoisomerase II Binding Protein 1 (TopBP1) binds the C-terminal region of Topo IIβ via its N-terminal region. In addition, TopBP1 contains a putative ADP-ribosylation site, two N-terminal NLS domains, and 8 repeating BRCA1 C-terminal (BRCT) domains found in DNA repair proteins, such as BRCA1, XRCC1, and Rad4. The BRCT domains of TopBP1 have been shown to bind DNA breaks, DNA nicks, and DNA termini, but not circular intact DNA. Thus, TopBP1 may localize topoisomerases to sites of DNA damage.

611874 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611874 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Yamane K, Katayama E, Tsuruo T. The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature. Biochem Biophys Res Commun. 2000; 279(2):678-684. (Biology). View Reference
  2. Yamane K, Kawabata M, Tsuruo T. A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator. Eur J Biochem. 1997; 250(3):794-799. (Biology). View Reference
  3. Yamane K, Tsuruo T. Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA. Oncogene. 1999; 18(37):5194-5203. (Biology). View Reference
611874 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.