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Purified Mouse Anti-Human DFF45
Purified Mouse Anti-Human DFF45

Western blot analysis of DFF45 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) .  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti-human DFF45 antibody.

Western blot analysis of DFF45 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152) .  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti-human DFF45 antibody.

Product Details
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BD Transduction Laboratories™
DNA Fragmentation Factor-45
Human (QC Testing)
Mouse IgG1
Human DFF45 aa. 105-305
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
45 kDa
250 µg/ml
AB_398349
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611036 Rev. 1
Antibody Details
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19/DFF45

Apoptosis, a selective process of genetically programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptotic cells are characterized by loss of cell volume, plasma membrane blebbing, nuclear condensation, chromatin aggregation, and endonucleocytic degradation of DNA into nucleosomal fragments. Caspase-3 (CPP32, Yama, apopain) is the most extensively studied apoptotic protein. An apoptotic signal induces the intracellular cleavage of caspase-3 from an inactive proform to an active fragment. The active form of caspase-3 cleaves several other apoptotic proteins including DNA Fragmentation Factor (DFF). DFF is thought to be either directly or indirectly responsible for the DNA laddering that is a hallmark of apoptosis. It is a heterodimer of 40 kDa (DFF40) and 45 kDa (DFF45) subunits. Cleavage of DFF45 into three fragments is a prerequisite for DFF-mediated DNA  fragmentation. Identity of the DFF fragment that mediates DNA laddering remains unclear. However, it is clear that DFF functions downstream of caspase-3 and is essential for the characteristic fragmentation of DNA during apoptosis.

611036 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611036 Rev.1
Citations & References
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Development References (5)

  1. Fulda S, Meyer E, Debatin KM. Inhibition of TRAIL-induced apoptosis by Bcl-2 overexpression. Oncogene. 2002; 21(15):2283-2294. (Biology: Western blot). View Reference
  2. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998; 95(9):2526-2533. (Biology). View Reference
  3. Li J, Chen P, Sinogeeva N, et al. Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells. J Biol Chem. 2002; 277(51):49504-49510. (Biology: Western blot). View Reference
  4. Liu X, Li P, Widlak P. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci U S A. 1998; 95(15):8461-8466. (Biology). View Reference
  5. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell. 1997; 89(2):175-184. (Biology). View Reference
View All (5) View Less
611036 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.