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Purified Mouse Anti- eIF-6
Purified Mouse Anti- eIF-6

Western blot analysis of eIF-6 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- eIF-6 antibody.

Purified Mouse Anti- eIF-6

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Western blot analysis of eIF-6 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- eIF-6 antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Product Details
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BD Transduction Laboratories™
Eukaryotic Initiation Factor-6
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human eIF-6 aa. 17-128
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
27 kDa
250 µg/ml
AB_398431
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611120 Rev. 1
Antibody Details
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23/eIF-6

Initiation of eukaryotic translation involves a series of reactions mediated by multiple eukaryotic initiation factors (eIFs).  As a prerequisite to initiation, the initiator methionyl-tRNA (Met-tRNAf), in a ternary complex with eIF-2 and GTP, binds to the 40S ribosomal subunit.  This 40S preinitiation complex is positioned at the initiation AUG codon to form the 40S initiation complex.  This complex is joined by the 60S ribosomal subunit to form the 80S initiation complex, which functions as a peptidyl transferase.  Once translation is completed, the polysomal complex is dismantled with the formation of the free 40S and 60S subunits.  eIF-6 is a monomeric protein which binds to the 60S ribosomal subunit and blocks its association with the 40S subunit.  The human eIF-6 is expressed in a wide range of tissues and exhibits a significant homology with sequences from S. cerevisiae and Drosophila.  The joining of the 60S subunit to the 40S initiation complex requires eIF-5; thus, eIF-5 & -6 may maintain equilibrium between the individual subunits and the 80S complex.

611120 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611120 Rev.1
Citations & References
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Development References (1)

  1. Si K, Chaudhuri J, Chevesich J, Maitra U. Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6. Proc Natl Acad Sci U S A. 1997; 94(26):14285-14290. (Biology). View Reference
611120 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.