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Purified Mouse Anti-Csk
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat Csk aa. 1-156
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
50 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610080 Rev. 7
Antibody Details
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The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60[c-src], p59[fyn], p53/56[lyn], p56[lck], and p62[yes]. This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59[fyn] and p53/56[lyn]. These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.

610080 Rev. 7
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610080 Rev.7
Citations & References
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Development References (5)

  1. Bergman M, Mustelin T, Oetken C. The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity. EMBO J. 1992; 11(8):2919-2924. (Biology). View Reference
  2. Bénistant C, Bourgaux JF, Chapuis H, Mottet N, Roche S, Bali JP. The COOH-terminal Src kinase Csk is a tumor antigen in human carcinoma. Cancer Res. 2001; 61(4):1415-1420. (Clone-specific: Immunofluorescence, Western blot). View Reference
  3. Okada M, Nada S, Yamanashi Y, Yamamoto T, Nakagawa H. CSK: a protein-tyrosine kinase involved in regulation of src family kinases. J Biol Chem. 1991; 266(36):24249-24252. (Biology). View Reference
  4. Takayama Y, Tanaka S, Nagai K, Okada M. Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999; 274(4):2291-2297. (Clone-specific: Immunoprecipitation). View Reference
  5. Wan Y, Bence K, Hata A, Kurosaki T, Veillette A, Huang XY. Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling. J Biol Chem. 1999; 272(27):17209-17215. (Clone-specific: In vitro kinase assay, Western blot). View Reference
View All (5) View Less
610080 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.