ELISA Capture: Purified Mouse Anti-Human CD121a (IL1-RI) (Cat. No. 551388) is useful as a capture antibody for a sandwich ELISA for measuring human IL-1 receptor I protein levels. Purified Mouse Anti-Human CD121a (IL1-RI) can be paired with a biotinylated detecting antibody, and recombinant soluble human IL-1 receptor type I as the standard. Purified Mouse Anti-Human CD121a (IL1-RI) should be titrated between 1 - 4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human IL-1 receptor type I ranging from 3000 to 20 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: sCD14, CD40, CD40L, IL-1Ra, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, LT-α, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFβ, Trx