ELISA Detection: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody for a sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the D8402E8 antibody (Cat. No. 551218) as the capture antibody, and with recombinant mouse IL-9 as the standard. Biotinylated D9302C12 antibody should be titrated (0.5 - 2.0 µg/ml) to determine optimal concentration for ELISA detection. An appropriate range of concentrations of the mouse IL-9 standard for obtaining a linear standard curve is 4 ng/ml to 30 pg/ml. For maximal sensitivity, BD Biosciences recommends an overnight incubation (4°C) of samples/standards with the coated capture antibody. For specific methodology, please visit our website, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.
This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Neutralization: The NA/LE format of this clone (Cat. No. 554472) is recommended for functional assays.