Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Norway (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      RY610 Mouse Anti-Mouse Siglec-G
      Product Details
      Down Arrow Up Arrow


      BD OptiBuild™
      Siglecg; mSiglec-G; Siglec10; Siglec 10; CD330
      Mouse (Tested in Development)
      Mouse IgG1, κ
      Mouse Extracellular Siglec-G Recombinant Protein
      Flow cytometry (Qualified)
      0.2 mg/ml
      243958
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. An isotype control should be used at the same concentration as the antibody of interest.
      10. CF™ is a trademark of Biotium, Inc.
      Show More blue down arrow Show Less blue up arrow
      758893 Rev. 2
      Antibody Details
      Down Arrow Up Arrow
      SH1

      The SH1 monoclonal antibody specifically binds to mouse Siglec-G (sialic acid binding immunoglobulin-like lectin G), also known as Siglec10. Siglec-G is a Type I transmembrane glycoprotein that belongs to the Immunoglobulin superfamily. Siglec-G functions as an adhesion molecule that mediates sialic-acid dependent binding to cells. Siglec-G is expressed on cells of the B cell lineage. Siglec-G is most highly expressed by pre-B cells and B1a cells within the B cell lineage and is not detectable on T cells. Siglec-G can inhibit B cell receptor-mediated calcium signaling when it is overexpressed. Mice that lack Siglec-G have significantly increased numbers of B1a cells that begins early in development and is B cell intrinsic. Siglec-G-deficient mice have higher titers of natural IgM antibodies than their normal counterparts. Mouse Siglec-G is the ortholog of human Siglec-10 (also known as, CD330).

      758893 Rev. 2
      Format Details
      Down Arrow Up Arrow
      RY610
      The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
      altImg
      RY610
      Yellow-Green 561 nm
      557 nm
      610 nm
      758893 Rev.2
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "758893" on CiteAb

      Development References (7)

      1. Aizawa H, Zimmermann N, Carrigan PE, Lee JJ, Rothenberg ME, Bochner BS. Molecular analysis of human Siglec-8 orthologs relevant to mouse eosinophils: identification of mouse orthologs of Siglec-5 (mSiglec-F) and Siglec-10 (mSiglec-G). Genomics. 2003; 82(5):521-530. (Biology). View Reference
      2. Hoffmann A, Kerr S, Jellusova J, et al. Siglec-G is a B1 cell-inhibitory receptor that controls expansion and calcium signaling of the B1 cell population. Nat Immunol. 2007; 8(7):695-704. (Biology). View Reference
      3. Hutzler S, Özgör L, Naito-Matsui Y, et al. The ligand-binding domain of Siglec-G is crucial for its selective inhibitory function on B1 cells.. J Immunol. 2014; 192(11):5406-14. (Immunogen: Flow cytometry). View Reference
      4. Jellusova J, Düber S, Gückel E, et al. Siglec-G regulates B1 cell survival and selection. J Immunol. 2010; 185(6):3277-3284. (Biology). View Reference
      5. Nitschke L. CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions. Immunol Rev. 2009; 230(1):128-143. (Biology). View Reference
      6. Nitschke L. Siglec-G is a B-1 cell inhibitory receptor and also controls B cell tolerance.. Ann N Y Acad Sci. 2015; 1362:117-21. (Biology). View Reference
      7. Özgör L, Meyer SJ, Korn M, Terörde K, Nitschke L. Sialic Acid Ligand Binding of CD22 and Siglec-G Determines Distinct B Cell Functions but Is Dispensable for B Cell Tolerance Induction.. J Immunol. 2018; 201(7):2107-2116. (Biology). View Reference
      View All (7) View Less
      758893 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.