Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- CF™ is a trademark of Biotium, Inc.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The ASC-8 monoclonal antibody specifically recognizes CD104 which is also known as Integrin beta 4 (Integrin β4 or β4 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions which are essential for cellular adhesion and migration as well as for regulating cellular growth and survival. CD104 (Integrin β4) is an ~205 kDa single-pass type I transmembrane glycoprotein that is encoded by ITGB4 (Integrin subunit beta 4). CD104 (Integrin β4) noncovalently associates with CD49f (Integrin α6 chain) to form the Integrin α6/β4 (CD49f/CD104) complex. CD104 (Integrin β4) is expressed on basal epithelial cells, Schwann cells, and some endothelial cells and tumor cells. The CD49f/CD104 complex serves as an adhesion receptor that binds to laminins and keratin filaments. It is a component of epidermal hemidesmosomes that facilitate the stable adhesion of basal epithelial cells to the underlying basement membrane.
Development References (10)
-
Egles C, Huet HA, Dogan F, et al. Integrin-blocking antibodies delay keratinocyte re-epithelialization in a human three-dimensional wound healing model.. PLoS One. 2010; 5(5):e10528. (Clone-specific: Flow cytometry, Functional assay). View Reference
-
Hemler ME, Crouse C, Sonnenberg A. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines. J Biol Chem. 1989; 264(11):6529-6535. (Biology). View Reference
-
Jones JC, Kurpakus MA, Cooper HM, Quaranta V. A function for the integrin alpha 6 beta 4 in the hemidesmosome. Cell Regul. 1991; 2(6):427-438. (Biology). View Reference
-
Kennel SJ, Epler RG, Lankford TK, et al. Second generation monoclonal antibodies to the human integrin alpha 6 beta 4. Hybridoma. 1990; 9(3):243-255. (Biology). View Reference
-
Mohri T, Adachi Y, Ikehara S, Hioki K, Tokunaga R, Taketani S. Activated Rac1 selectively up-regulates the expression of integrin alpha6beta4 and induces cell adhesion and membrane ruffles of nonadherent colon cancer Colo201 cells.. Exp Cell Res. 1999; 253(2):533-40. (Clone-specific: Flow cytometry, Functional assay). View Reference
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
-
Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS. Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.. Am J Pathol. 1996; 148(5):1445-61. (Immunogen: Flow cytometry). View Reference
-
Sonnenberg A, Calafat J, Janssen H, et al. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.. J Cell Biol. 1991; 113(4):907-17. (Biology). View Reference
-
Tamura RN, Rozzo C, Starr L, et al. Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4.. J Cell Biol. 1990; 111(4):1593-604. (Biology). View Reference
-
Tanaka Y, Adachi T, Gianocotti F. CD104 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:432-434.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.