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      RB705 Mouse Anti-Mouse Vβ 17[a] T-Cell Receptor
      Product Details
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      BD OptiBuild™
      T cell receptor beta variable 17[a]; TCR Vb17[a]
      Mouse (Tested in Development)
      Mouse BALB/c IgG2a, κ
      Mouse T cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      100124681
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      3. For U.S. patents that may apply, see bd.com/patents.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
      9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      10. An isotype control should be used at the same concentration as the antibody of interest.
      11. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
      12. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
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      757049 Rev. 1
      Antibody Details
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      KJ23

      The KJ23 monoclonal antibody specifically recognizes Vβ 17[a] T-cell Receptor (TCR) of mice having the a haplotype (eg,C57L, SJL, SWR) of the Tcrb gene complex. Strains having the b (eg, A, AKR, BALB/c, CBA,C3H/He, C57BL, C58, DBA/1, DBA/2) Tcrb haplotype do not express functional Vβ 17 TCR, and the Tcrb-V17 gene locus is deleted in mice having the c (eg, RIII) haplotype. Vβ 17[a] TCR-bearing Tlymphocytes are clonally eliminated in mice expressing I-E (eg, C57BR). KJ23 antibody also recognizes two phenotypic variants of the Vβ 17[a] TCR: Vβ 17[a2] expressed in a variety of wild-derivedmouse strains and Vβ 17[a(cz)] expressed in Mtv-free CZ mice. The effects of Mtv-encoded superantigens upon Vβ 17[a] TCR-bearing T cells has been reviewed. Plate-bound KJ23 antibody activates Vβ 17[a] TCR-bearing T cells, and injection of the antibody can deplete Vβ 17[a]-bearing Tcells.

      757049 Rev. 1
      Format Details
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      RB705
      The BD Horizon RealBlue™ 705 (RB705) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 707-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB705 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), RB705 can be used as an alternative to PerCP-Cy5.5 or BB700 and we recommend using an optical filter centered near 710-nm (e.g., a 695/40 or 710/50-nm bandpass filter). For spectral instruments equipped with a Blue laser (488-nm), it can be used in conjunction with PerCP-Cy5.5. RB705 is on average brighter than PerCP-Cy5.5 and BB700, and has minimal spillover into Yellow-Green detectors.
      altImg
      RB705
      Blue 488 nm
      498 nm
      707 nm
      757049 Rev.1
      Citations & References
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      View product citations for antibody "757049" on CiteAb

      Development References (8)

      1. Cazenave PA, Marche PN, Jouvin-Marche E, et al. V beta 17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the V beta 17 region greatly alter T cell receptor specificity. Cell. 1990; 63(4):717-728. (Biology). View Reference
      2. Haqqi TM, Banerjee S, Anderson GD, David CS. RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes. J Exp Med. 1989; 169(6):1903-1909. (Biology). View Reference
      3. Kappler JW, Roehm N, Marrack P. T cell tolerance by clonal elimination in the thymus. Cell. 1987; 49(2):273-280. (Biology). View Reference
      4. Kappler JW, Wade T, White J, . A T cell receptor V beta segment that imparts reactivity to a class II major histocompatibility complex product. Cell. 1987; 49(2):263-271. (Immunogen). View Reference
      5. Katz JD, Lebow LT, Bonavida B. The in vivo depletion of V beta 17a+ T cells results in the inhibition of reticulum cell sarcoma growth in SJL/J mice. Evidence for the use of anticlonotypic antibody therapy in the control of malignancy. J Immunol. 1989; 143(4):1387-1395. (Biology). View Reference
      6. Ramsdell F, Lantz T, Fowlkes BJ. A nondeletional mechanism of thymic self tolerance. Science. 1989; 246(4933):1038-1041. (Biology). View Reference
      7. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
      8. Wade T, Bill J, Marrack PC, Palmer E, Kappler JW. Molecular basis for the nonexpression of V beta 17 in some strains of mice. J Immunol. 1988; 141(6):2165-2167. (Biology). View Reference
      View All (8) View Less
      757049 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.