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      R718 Mouse Anti-Human Desmoglein 2
      R718 Mouse Anti-Human Desmoglein 2
      Flow cytometric analysis of Desmoglein 2 expression on viable Human CD34+ lymphoid cells.  Human peripheral blood mononuclear cells (PBMC) were stained with PE Mouse Anti-Human CD34 antibody (Cat. No. 555822) and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 570776/570854; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Desmoglein 2 expression (or Ig Isotype control staining) [Right Plot] was derived from gated CD34 positive cells [Left Plot as indicated] with the forward and side light-scatter characteristics of viable (DAPI-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.
      R718 Mouse Anti-Human Desmoglein 2
      Flow cytometric analysis of Desmoglein 2 expression on viable Human HeLa cells.  Cells from the HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 570776/570854; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing the expression of Desmoglein 2 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.
      R718 Mouse Anti-Human Desmoglein 2 R718 Mouse Anti-Human Desmoglein 2
      Flow cytometric analysis of Desmoglein 2 expression on viable Human CD34+ lymphoid cells.  Human peripheral blood mononuclear cells (PBMC) were stained with PE Mouse Anti-Human CD34 antibody (Cat. No. 555822) and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 570776/570854; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Desmoglein 2 expression (or Ig Isotype control staining) [Right Plot] was derived from gated CD34 positive cells [Left Plot as indicated] with the forward and side light-scatter characteristics of viable (DAPI-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.
      Flow cytometric analysis of Desmoglein 2 expression on viable Human HeLa cells.  Cells from the HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 566928; dashed line histogram) or BD Horizon™ R718 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 570776/570854; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing the expression of Desmoglein 2 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD FACSymphony™ A5 SE Cell Analyzer System and FlowJo™ Software.
      Product Details
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      BD Horizon™
      DSG2; CDHF5; Cadherin family member 5; Desmoglein-2; desmoglein-2
      Human (QC Testing)
      Mouse IgG1, κ
      Human Full-length recomb. Desmoglein 2
      Flow cytometry (Routinely Tested)
      5 µl/test
      1829
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. For U.S. patents that may apply, see bd.com/patents.
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      570854 Rev. 1
      Antibody Details
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      6D8

      The 6D8 monoclonal antibody specifically recognizes the extracellular region of human Desmoglein 2 which is also known as Cadherin family member 5 (CDHF5). Desmoglein 2 is a type 1 transmembrane glycoprotein encoded by DSG2 which belongs to the cadherin cell adhesion molecule superfamily. Desmoglein 2 is variably expressed by epithelial cells, cardiomyocytes, endothelial progenitors associated with neoangiogenesis, and by CD34+CD45lo hematopoietic stem and progenitor cells. Desmoglein-2 plays essential roles in cellular adhesion, migration, proliferation, apoptosis and intracellular signaling. This calcium-dependent adhesion molecule is an essential component of  intercellular desmosome junctions. These adhesive desmosomal junctions serve to link adjacent cells together such as in the formation of the intestinal epithelial barrier. The desmosomes can also connect with the cytoskeleton through interactions with cytoplasmic plaque proteins and intermediate filaments. Desmoglein 2 can also serve as a receptor for adenoviruses.

      570854 Rev. 1
      Format Details
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      570854 Rev.1
      Citations & References
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      View product citations for antibody "570854" on CiteAb

      Development References (5)

      1. Ebert LM, Tan LY, Johan MZ, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.. Angiogenesis. 2016; 19(4):463-86. (Clone-specific: Flow cytometry). View Reference
      2. Ebert LM, Vandyke K, Johan MZ, et al. Desmoglein-2 expression is an independent predictor of poor prognosis patients with multiple myeloma.. Mol Oncol. 2022; 16(6):1221-1240. (Clone-specific: Flow cytometry). View Reference
      3. Tan LY, Mintoff C, Johan MZ, et al. Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome.. Oncotarget. 2016; 7(29):46492-46508. (Clone-specific: Flow cytometry). View Reference
      4. Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.. J Cell Sci. 1996; 109 ( Pt 5):1143-54. (Immunogen: Immunoprecipitation, Radioimmunoassay). View Reference
      5. Wang H, Li ZY, Liu Y, et al. Desmoglein 2 is a receptor for adenovirus serotypes 3, 7, 11 and 14.. Nat Med. 2011; 17(1):96-104. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
      View All (5) View Less
      570854 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.