BD Pharmingen™ PE Mouse anti-Human CD160
Clone BY55 (RUO)
Flow cytometric analysis CD160 expression on human peripheral blood lymphocytes. Human whole blood cells were stained with APC Mouse Anti-Human CD56 (Cat. No. 555518) and either PE Mouse IgM, κ Isotype Control (Cat. No. 555584; Left Panel) or PE Mouse anti-Human CD160 (Cat. No. 562118; Right Panel). After incubation the erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plots showing the correlated expression of CD160 or background Ig isotype control staining versus CD56 were derived from gated events of the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
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Flow cytometric analysis CD160 expression on human peripheral blood lymphocytes. Human whole blood cells were stained with APC Mouse Anti-Human CD56 (Cat. No. 555518) and either PE Mouse IgM, κ Isotype Control (Cat. No. 555584; Left Panel) or PE Mouse anti-Human CD160 (Cat. No. 562118; Right Panel). After incubation the erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The two-color dot plots showing the correlated expression of CD160 or background Ig isotype control staining versus CD56 were derived from gated events of the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
Product Details
BD Pharmingen™
BY55; BY55 antigen; NK1; NK28
Human (QC Testing)
Mouse BALB/c IgM
Human YT2C2 NK Cell Line
Flow cytometry (Routinely Tested)
5 µl
VII
11126
AB_10894019
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
PE Mouse IgM, κ Isotype Control RUO
Size
100 Tests
Cat No.
555584
APC Mouse Anti-Human CD56 (NCAM-1) RUO
Size
100 Tests
Cat No.
555518
562118 Rev. 1
Antibody Details
BY55
The BY55 monoclonal antibody specifically binds to the human cell surface molecule, CD160 (also known as BY55). CD160 is a glycosyolphosphatidylinositol-anchored glycoprotein member of the immunoglobulin superfamily. It is expressed on cytotoxic αβ CD8+ T cells and natural killer (NK) cells, γδ T cells and intestinal intraepithelial T lymphocytes. CD160 can bind to classical and nonclassical MHC class I molecules. This interaction can costimulate the proliferation of activated human T lymphocytes including CD28-negative T lymphocytes.
562118 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
562118 Rev.1
Citations & References
View product citations for antibody "562118" on CiteAb
Development References (3)
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Agrawal S, Marquet J, Freeman GJ, et al. Cutting edge: MHC class I triggering by a novel cell surface ligand costimulates proliferation of activated human T cells. J Immunol. 1999; 162(3):1223-1226. (Clone-specific: Flow cytometry). View Reference
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Anumanthan A, Bensussan A, Boumsell L, et al. Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. J Immunol. 1998; 161(6):2780-2790. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
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Maiza H, Leca G, Mansur IG, Schiavon V, Boumsell L, Bensussan A. A novel 80-kD cell surface structure identifies human circulating lymphocytes with natural killer activity. J Exp Med. 1993; 178(3):1121-1126. (Clone-specific: Flow cytometry). View Reference
562118 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.