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Flow cytometric analysis of CD118 (LIFR) expression on human JAR cells. Cells from the JAR (Choriocarcinoma, ATCC HTB-144) cell line were stained with 1 μg of either PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dotted line histogram) or PE Anti-Human CD118 (LIFR) antibody (Cat. No. 566384; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The histogram showing CD118 (LIFR) expression (or Ig Isotype control staining) were derived from 7-AAD negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
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BD Pharmingen™ PE Mouse Anti-Human CD118 (LIFR)
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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The 12D3 monoclonal antibody specifically recognizes CD118 which is also known as the Leukemia Inhibitory Factor Receptor (LIF Receptor or LIFR). CD118 is a type I transmembrane glycoprotein that is encoded by LIFR (leukemia inhibitory factor receptor alpha) and belongs to the type 1 cytokine receptor family. CD118 associates with CD130 (gp130) to form the functional LIF Receptor. The CD118/CD130 complex is expressed by a wide variety of cells including stem cells, hepatocytes, adipocytes, myoblasts, neural and glial cells. LIF and Oncostatin M can both act through the CD118/CD130 heterodimer to regulate cellular differentiation, survival and proliferation in developing and adult individuals.

Development References (3)
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Bitard J, Daburon S, Duplomb L, et al. Mutations in the immunoglobulin-like domain of gp190, the leukemia inhibitory factor (LIF) receptor, increase or decrease its affinity for LIF.. J Biol Chem. 2003; 278(18):16253-61. (Clone-specific: Western blot). View Reference
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Lelièvre E, Plun-Favreau H, Chevalier S, et al. Signaling pathways recruited by the cardiotrophin-like cytokine/cytokine-like factor-1 composite cytokine: specific requirement of the membrane-bound form of ciliary neurotrophic factor receptor alpha component.. J Biol Chem. 2001; 276(25):22476-84. (Biology). View Reference
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Taupin JL, Miossec V, Pitard V, et al. Binding of leukemia inhibitory factor (LIF) to mutants of its low affinity receptor, gp190, reveals a LIF binding site outside and interactions between the two cytokine binding domains.. J Biol Chem. 1999; 274(20):14482-9. (Immunogen). View Reference
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