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PE Mouse Anti-Human CD118 (LIFR)
PE Mouse Anti-Human CD118 (LIFR)
Flow cytometric analysis of CD118 (LIFR) expression on human JAR cells.  Cells from the JAR (Choriocarcinoma, ATCC HTB-144) cell line were stained with 1 μg of either PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dotted line histogram) or PE Anti-Human CD118 (LIFR) antibody (Cat. No. 566384; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The histogram showing CD118 (LIFR) expression (or Ig Isotype control staining) were derived from 7-AAD negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CD118 (LIFR) expression on human JAR cells.  Cells from the JAR (Choriocarcinoma, ATCC HTB-144) cell line were stained with 1 μg of either PE Mouse IgG1, κ Isotype Control (Cat. No.554680; dotted line histogram) or PE Anti-Human CD118 (LIFR) antibody (Cat. No. 566384; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The histogram showing CD118 (LIFR) expression (or Ig Isotype control staining) were derived from 7-AAD negative-gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
LIF Receptor; LIFR; LIF-R; SWS; SJS2; STWS
Human (QC Testing)
Mouse IgG1, κ
Human LIFR Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739720
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566384 Rev. 1
Antibody Details
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12D3

The 12D3 monoclonal antibody specifically recognizes CD118 which is also known as the Leukemia Inhibitory Factor Receptor (LIF Receptor or LIFR). CD118 is a type I transmembrane glycoprotein that is encoded by LIFR (leukemia inhibitory factor receptor alpha) and belongs to the type 1 cytokine receptor family. CD118 associates with CD130 (gp130) to form the functional LIF Receptor. The CD118/CD130 complex is expressed by a wide variety of cells including stem cells, hepatocytes, adipocytes, myoblasts, neural and glial cells. LIF and Oncostatin M can both act through the CD118/CD130 heterodimer to regulate cellular differentiation, survival and proliferation in developing and adult individuals.

        

566384 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566384 Rev.1
Citations & References
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View product citations for antibody "566384" on CiteAb

Development References (3)

  1. Bitard J, Daburon S, Duplomb L, et al. Mutations in the immunoglobulin-like domain of gp190, the leukemia inhibitory factor (LIF) receptor, increase or decrease its affinity for LIF.. J Biol Chem. 2003; 278(18):16253-61. (Clone-specific: Western blot). View Reference
  2. Lelièvre E, Plun-Favreau H, Chevalier S, et al. Signaling pathways recruited by the cardiotrophin-like cytokine/cytokine-like factor-1 composite cytokine: specific requirement of the membrane-bound form of ciliary neurotrophic factor receptor alpha component.. J Biol Chem. 2001; 276(25):22476-84. (Biology). View Reference
  3. Taupin JL, Miossec V, Pitard V, et al. Binding of leukemia inhibitory factor (LIF) to mutants of its low affinity receptor, gp190, reveals a LIF binding site outside and interactions between the two cytokine binding domains.. J Biol Chem. 1999; 274(20):14482-9. (Immunogen). View Reference
566384 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.