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FITC Mouse Anti-Rat CD86
Product Details
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BD Pharmingen™
Cd86; B7-2
Rat (QC Testing)
Mouse BALB/c IgG1, κ
HTLV-1-transformed rat T-cell line Lewis-S1
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555018 Rev. 5
Antibody Details
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The 24F antibody reacts with CD86 (B7-2), a member of the Ig superfamily of transmembrane proteins. CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD86 is predominantly expressed on antigen-presenting cells. It can be upregulated on splenic B cells by in vitro polyclonal activation, such as LPS treatment. 24F mAb is reported to block the costimulatory function of rat CD86 and to immunoprecipitate CD86 from Lewis-S1 cell lysates.HTLV-1-transformed rat T-cell line Lewis-S1

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555018 Rev. 5
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
555018 Rev.5
Citations & References
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Development References (3)

  1. Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology). View Reference
  2. Damoiseaux JG, Yagita H, Okumura K, van Breda Vriesman PJ. Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells. J Leukoc Biol. 1998; 64(6):803-809. (Clone-specific: Immunohistochemistry). View Reference
  3. Maeda K, Sato T, Azuma M, Yagita H, Okumura K. Characterization of rat CD80 and CD86 by molecular cloning and mAb. Int Immunol. 1997; 9(7):993-1000. (Immunogen: (Co)-stimulation, Flow cytometry, Immunoprecipitation). View Reference
555018 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.