Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The 6D8 monoclonal antibody specifically recognizes the extracellular region of human Desmoglein 2 which is also known as Cadherin family member 5 (CDHF5). Desmoglein 2 is a type 1 transmembrane glycoprotein encoded by DSG2 which belongs to the cadherin cell adhesion molecule superfamily. Desmoglein 2 is variably expressed by epithelial cells, cardiomyocytes, endothelial progenitors associated with neoangiogenesis, and by CD34+CD45lo hematopoietic stem and progenitor cells. Desmoglein-2 plays essential roles in cellular adhesion, migration, proliferation, apoptosis and intracellular signaling. This calcium-dependent adhesion molecule is an essential component of intercellular desmosome junctions. These adhesive desmosomal junctions serve to link adjacent cells together such as in the formation of the intestinal epithelial barrier. The desmosomes can also connect with the cytoskeleton through interactions with cytoplasmic plaque proteins and intermediate filaments. Desmoglein 2 can also serve as a receptor for adenoviruses.
Development References (5)
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Ebert LM, Tan LY, Johan MZ, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.. Angiogenesis. 2016; 19(4):463-86. (Clone-specific: Flow cytometry). View Reference
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Ebert LM, Vandyke K, Johan MZ, et al. Desmoglein-2 expression is an independent predictor of poor prognosis patients with multiple myeloma.. Mol Oncol. 2022; 16(6):1221-1240. (Clone-specific: Flow cytometry). View Reference
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Tan LY, Mintoff C, Johan MZ, et al. Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome.. Oncotarget. 2016; 7(29):46492-46508. (Clone-specific: Flow cytometry). View Reference
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Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.. J Cell Sci. 1996; 109 ( Pt 5):1143-54. (Immunogen: Immunoprecipitation, Radioimmunoassay). View Reference
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Wang H, Li ZY, Liu Y, et al. Desmoglein 2 is a receptor for adenovirus serotypes 3, 7, 11 and 14.. Nat Med. 2011; 17(1):96-104. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.