BD OptiBuild™ BV711 Rat Anti-Mouse Folate Receptor β (FRβ)
Clone 10/FR-β (also known as 10/FR2; Clone 10/FR2; CL10)
(RUO)
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
The 10/FR-β monoclonal antibody (aka, Clone 10/FR2 or CL10) specifically recognizes mouse Folate Receptor beta (Folate Receptor β or FRβ), a member of the folate receptor family. This cell surface receptor is a glycosylphosphatidylinositol (GPI)-linked membrane protein that is encoded by Folr2 which belongs to the folate-binding receptor family. The Folate Receptor β (FRβ) binds to folate and folic acid derivatives and transports them into cells that are essential for methionine and DNA synthesis which support cell growth and proliferation. Folate Receptor β (FRβ) is expressed on placental cells and on monocytes as well as macrophages found in normal and diseased tissues, eg, tumor-associated macrophages (TAMs) in tumors. Soluble forms of Folate Receptor β (FRβ) have also been described which can be found in various body fluids.
Development References (5)
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Nagai T, Tanaka M, Tsuneyoshi Y, et al. Targeting tumor-associated macrophages in an experimental glioma model with a recombinant immunotoxin to folate receptor beta.. Cancer Immunol Immunother. 2009; 58(10):1577-86. (Immunogen: Flow cytometry). View Reference
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Rodriguez-Garcia A, Lynn RC, Poussin M, et al. CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy.. Nat Commun. 2021; 12(1):877. (Clone-specific: Flow cytometry). View Reference
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Roy AG, Robinson JM, Sharma P, et al. Folate Receptor Beta as a Direct and Indirect Target for Antibody-Based Cancer Immunotherapy.. Int J Mol Sci. 2021; 22(11):5572. (Clone-specific: Flow cytometry). View Reference
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Tie Y, Zheng H, He Z, et al. Targeting folate receptor β positive tumor-associated macrophages in lung cancer with a folate-modified liposomal complex.. Signal Transduct Target Ther. 2020; 5(1):6. (Clone-specific: Flow cytometry). View Reference
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Utz SG, See P, Mildenberger W, et al. Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development.. Cell. 2020; 181(3):557-573.e18. (Clone-specific: Flow cytometry). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.