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      BV421 Rat Anti-Mouse Treml4
      BV421 Rat Anti-Mouse Treml4
      Flow cytometric analysis of Treml4 expression on viable Mouse splenic leukocytes.  C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD8a (Cat No. 563786) and APC Hamster Anti-Mouse CD11c (Cat No. 550261) antibodies, and with either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse TremI4 antibody (Cat. No. 569806/569880; solid line histogram) at 1 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The fluorescence histogram showing the expression of Treml4 (or Ig Isotype control staining) was derived from CD8a and CD11c-positive gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Rat Anti-Mouse Treml4
      Flow cytometric analysis of Treml4 expression on viable Mouse splenic leukocytes.  C57BL/6 Mouse splenocytes were treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD Horizon™ BUV395 Rat Anti-Mouse CD8a (Cat No. 563786) and APC Hamster Anti-Mouse CD11c (Cat No. 550261) antibodies, and with either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse TremI4 antibody (Cat. No. 569806/569880; solid line histogram) at 1 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The fluorescence histogram showing the expression of Treml4 (or Ig Isotype control staining) was derived from CD8a and CD11c-positive gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      Treml4; TLT-4; TLT4; IDCP1
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG1, κ
      Recombinant Mouse TREML4 Extracellular Domain-Human IgG1 Fc
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      224840
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
      7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. For U.S. patents that may apply, see bd.com/patents.
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      569806 Rev. 1
      Antibody Details
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      16E5

      The 16E5 monoclonal antibody specifically recognizes Trem-like 4 (Treml4) which is also known as Trem-like transcript 4 protein (TLT-4 or TLT4).  Treml4 is a type I transmembrane glycoprotein protein that is encoded by Treml4 (Treml4 triggering receptor expressed on myeloid cells-like 4). Treml4 is comprised of a single IgV-like domain in its extracellular region that is followed by a transmembrane segment and a short cytoplasmic tail. Treml4 is expressed on splenic macrophages and dendritic cells (DC), particularly CD8+ dendritic cells. Treml4 can associate with DAP12 and regulate immune responses. It reportedly binds to B7-H3 and to apoptotic or necrotic cells.

      569806 Rev. 1
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      569806 Rev.1
      Citations & References
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      View product citations for antibody "569806" on CiteAb

      Development References (3)

      1. Hemmi H, Idoyaga J, Suda K, et al. A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.. J Immunol. 2009; 182(3):1278-86. (Immunogen: Flow cytometry, Western blot). View Reference
      2. Hemmi H, Zaidi N, Wang B, et al. Treml4, an Ig superfamily member, mediates presentation of several antigens to T cells in vivo, including protective immunity to HER2 protein.. J Immunol. 2012; 188(3):1147-55. (Clone-specific: Flow cytometry). View Reference
      3. Pek RH, Yuan X, Rietzschel N, et al. Hemozoin produced by mammals confers heme tolerance.. Elife. 2019; 8:e49503. (Clone-specific: Flow cytometry). View Reference
      569806 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.