BV421 Mouse Anti-Human uPA (PLAU)

BD Horizon™ BV421 Mouse Anti-Human uPA (PLAU)

Name: BV421 Mouse Anti-Human uPA (PLAU)
Brand: BV421 Mouse Anti-Human uPA (PLAU)
SKU: 570389

Clone UK98/6 (also known as UK 98/6; 98/6) (RUO)

Flow cytometric analysis of uPA (PLAU) expression by Human Ramos cells or HT-1080 cells. Cells from the Human Ramos (Burkitt Lymphoma, ATCC® CRL-1596™) [Left Plot] or Human HT-1080 (Fibrosarcoma, ATCC® CCL-121™) [Right Plot] cell lines were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220). The cells were then stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histograms) or BD Horizon™ BV421 Mouse Anti-Human uPA (PLAU) antibody (Cat. No. 570389/570459; solid line histograms). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histograms showing uPA (PLAU) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Horizon™

Alternative Name: ATF; PLAU; U-plasminogen activator; UPA; u-PA; urokinase-type plasminogen activator

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Purified Human Urokinase-type Plasminogen Activator

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 5328

RRID: AB_3685706

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Brilliant Stain Buffer RUO

Size 1000 Tests Cat No. 566349

Brilliant Stain Buffer Plus RUO

Size 1000 Tests Cat No. 566385

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

BV421 Mouse IgG1, k Isotype Control RUO

Size 50 µg Cat No. 562438

View Details

570389 Rev. 1

Antibody Details

UK98/6

The UK98/6 monoclonal antibody specifically recognizes Urokinase-type Plasminogen Activator (uPA) that is encoded by PLAU (Plasminogen Activator Urokinase). uPA (PLAU) is secreted as an inactive single-chain precursor protein (Pro-urokinase or Pro-uPA) by various cells including vascular endothelial cells, renal epithelial cells, fibroblasts, monocytes, macrophages, smooth muscle cells and tumor cells of different origin. Following proteolytic cleavage, two resulting chains are disulfide bonded together with an amino-terminal A-chain (ATF) with EGF-like growth factor and Kringle domains bound to a catalytically active peptidase, the C-terminal B-chain. uPA (PLAU) binds to cell-surface CD87 (also known as, Urokinase Plasminogen Activator Receptor or uPA-R) by its growth factor-like domain. CD87 is widely expressed by monocytes, neutrophils, dendritic cells, activated T cells, NK cells, fibroblasts, endothelial cells, keratinocytes, and some tumor cells. The cell-associated form of uPA (PLAU) efficiently acts upon the plasminogen proenzyme to generate active plasmin which degrades blood plasma proteins and dissolves fibrin blood clots. uPA (PLAU) also activates collagenases that are involved in the breakdown of the extracellular matrix as well as some mediators of the complement system. It may play a role in tumor cell proliferation, migration, and metastases. A specific polymorphism of uPA (PLAU) is associated with late-onset Alzheimer's disease. The UK98-6 antibody can reportedly suppress the biological activity of uPA (PLAU).

570389 Rev. 1

Format Details

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV421

Excitation Source: Violet 405 nm

Excitation Max: 407 nm

Emission Max: 423 nm

570389 Rev.1

Citations & References

View product citations for antibody "570389" on CiteAb

Development References (6)

Danø K, Andreasen PA, Grøndahl-Hansen J, Kristensen P, Nielsen LS, Skriver L. Plasminogen activators, tissue degradation, and cancer.. Adv Cancer Res. 1985; 44:139-266. (Biology). View Reference
Finckh U, van Hadeln K, Müller-Thomsen T, et al. Association of late-onset Alzheimer disease with a genotype of PLAU, the gene encoding urokinase-type plasminogen activator on chromosome 10q22.2.. Neurogenetics. 2003; 4(4):213-7. (Biology). View Reference
Heidtmann HH, Hofmann M, Jacob E, Erbil C, Havemann K, Schwartz-Albiez R. Synthesis and secretion of plasminogen activators and plasminogen activator inhibitors in cell lines of different groups of human lung tumors.. Cancer Res. 1989; 49(24 Pt 1):6960-5. (Biology: Flow cytometry). View Reference
Meissauer A, Kramer MD, Hofmann M, et al. Urokinase-type and tissue-type plasminogen activators are essential for in vitro invasion of human melanoma cells.. Exp Cell Res. 1991; 192(2):453-9. (Clone-specific: Blocking, ELISA, Functional assay). View Reference
Meissauer A, Kramer MD, Schirrmacher V, Brunner G. Generation of cell surface-bound plasmin by cell-associated urokinase-type or secreted tissue-type plasminogen activator: a key event in melanoma cell invasiveness in vitro.. Exp Cell Res. 1992; 199(2):179-90. (Clone-specific: Blocking, ELISA, Functional assay). View Reference
Schwartz-Albiez R, Heidtmann HH, Wolf D, Schirrmacher V, Moldenhauer G. Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase.. Br J Cancer. 1992; 65(1):51-7. (Immunogen: ELISA, Radioimmunoassay, Western blot). View Reference

View All (6)

570389 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.