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BUV615 Rat Anti-Mouse CD56 (NCAM-1)
Product Details
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BD OptiBuild™
CD56; E-NCAM; N-CAM-1; Ncam; Ncam1
Mouse (Tested in Development)
Rat IgG2a, κ
Mouse NCAM-1 Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).

Product Notices

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Researchers should determine the optimal concentration of this reagent for their individual applications.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  6. Please refer to for technical protocols.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Please refer to to access safety data sheets (SDS).
  9. CF™ is a trademark of Biotium, Inc.
  10. BD Horizon Brilliant Ultraviolet 615 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
751046 Rev. 2
Antibody Details
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The 809220 monoclonal antibody specifically recognizes CD56 which is also known as Neural cell adhesion molecule 1 (NCAM-1), Neural cell adhesion molecule (NCAM), or embryonic NCAM (E-NCAM). CD56 (NCAM-1) is a 120-180 kDa type I transmembrane glycoprotein that is encoded by Ncam1 which belongs to the immunoglobulin superfamily (IgSF). Three different isoforms of CD56 (NCAM-1) have been described that vary in their cytoplasmic domains. The 180 kDa long isoform contains five consecutive IgC-like domains followed by two fibronectin type III domains in its extracellular region, a transmembrane sequence and a large cytoplasmic domain. A 140 kDa isoform has a shorter cytoplasmic tail whereas the 120 kDa isoform is glycophosphatidylinositol (GPI)-linked to the cell membrane. CD56 (NCAM-1) functions on cells as a hemophilic or heterophilic adhesion molecule and as a receptor for various ligands including certain growth factors. Throughout development and adulthood, CD56 (NCAM-1) is expressed on various cell types such as neurons where it might play roles in cellular migration, axonal guidance, and synapse formation. It can also be expressed on astrocytes as well as bone marrow neutrophils and some monocytes and might be involved in cellular migration and other functions. Polysialylation of CD56 (NCAM-1) reduces its ability to dimerize and can thereby affect the functions of cells that express it.

The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP.  Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).

751046 Rev. 2
Format Details
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The BD Horizon Brilliant™ Ultraviolet 615 (BUV615) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. BUV615, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 615-nm (e.g, 610/20 bandpass filter). The acceptor dye can be excited by the Blue (488-nm) and yellow-green (561-nm) lasers resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Ultraviolet 355 nm
350 nm
615 nm
751046 Rev.2
Citations & References
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Development References (4)

  1. Fujita T, Chen MJ, Li B, et al. Neuronal transgene expression in dominant-negative SNARE mice.. J Neurosci. 2014; 34(50):16594-604. (Clone-specific: Fluorescence activated cell sorting). View Reference
  2. Li S, Nie EH, Yin Y, et al. GDF10 is a signal for axonal sprouting and functional recovery after stroke.. Nat Neurosci. 2015; 18(12):1737-45. (Clone-specific: Fluorescence activated cell sorting). View Reference
  3. Rougon G, Deagostini-Bazin H, Hirn M, Goridis C. Tissue- and developmental stage-specific forms of a neural cell surface antigen linked to differences in glycosylation of a common polypeptide.. EMBO J. 1982; 1(10):1239-44. (Biology). View Reference
  4. Stamatos NM, Zhang L, Jokilammi A, et al. Changes in polysialic acid expression on myeloid cells during differentiation and recruitment to sites of inflammation: role in phagocytosis. Glycobiology. 2014; 24(9):864-879. (Biology). View Reference
View All (4) View Less
751046 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.