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BUV615 Rat Anti-Mouse CD2
Product Details
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BD OptiBuild™
LFA-2; LFA-3 receptor; Ly37; Ly-37; Lymphocyte antigen 37; T11
Mouse (Tested in Development)
Rat SD, also known as Sprague-Dawley (outbred) IgG2b, λ
Mouse BALB/c Thymocytes
Flow cytometry (Qualified)
0.2 mg/ml
12481
AB_2875167
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Note:  When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed.  For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).

Product Notices

  1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  2. Researchers should determine the optimal concentration of this reagent for their individual applications.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. CF™ is a trademark of Biotium, Inc.
  10. BD Horizon Brilliant Ultraviolet 615 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
751141 Rev. 2
Antibody Details
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RM2-5

The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2). CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse. CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation. In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes. CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes. In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells. The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion.

The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP.  Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).

751141 Rev. 2
Format Details
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BUV615
The BD Horizon Brilliant™ Ultraviolet 615 (BUV615) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. BUV615, driven by BD innovation, is designed to be excited by the ultraviolet laser (355 nm) and detected using an optical filter centered near 615-nm (e.g, 610/20 bandpass filter). The acceptor dye can be excited by the Blue (488-nm) and yellow-green (561-nm) lasers resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV615
Ultraviolet 355 nm
350 nm
615 nm
751141 Rev.2
Citations & References
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Development References (16)

  1. Ayroldi E, Migliorati G, Cannarile L, Moraca R, Delfino DV, Riccardi C. CD2 rescues T cells from T-cell receptor/CD3 apoptosis: a role for the Fas/Fas-L system. Blood. 1997; 89(10):3717-3726. (Biology). View Reference
  2. Cibotti R, Punt JA, Dash KS, Sharrow SO, Singer A. Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals. Immunity. 1997; 6(3):245-255. (Clone-specific: Induction). View Reference
  3. Criado G, Feito MJ, Rojo JM. CD4-dependent and -independent association of protein tyrosine kinases to the T cell receptor/CD3 complex of CD4+ mouse T lymphocytes. Eur J Immunol. 1996; 26(6):1228-1234. (Clone-specific: Immunoprecipitation). View Reference
  4. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
  5. Hayday A, Theodoridis E, Ramsburg E, Shires J. Intraepithelial lymphocytes: exploring the Third Way in immunology. Nat Immunol. 2001; 2(11):997-1003. (Biology). View Reference
  6. Kato K, Koyanagi M, Okada H, et al. CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation. J Exp Med. 1992; 176(5):1241-1249. (Biology). View Reference
  7. Kuo S, El Guindy A, Panwala CM, Hagan PM, Camerini V. Differential appearance of T cell subsets in the large and small intestine of neonatal mice. Pediatr Res. 2001; 49(4):543-551. (Biology). View Reference
  8. Masten BJ, Yates JL, Pollard Koga AM, Lipscomb MF. Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L. Am J Respir Cell Mol Biol. 1997; 16(3):335-342. (Clone-specific: Blocking). View Reference
  9. Nakamura T, Takahashi K, Fukazawa T, et al. Relative contribution of CD2 and LFA-1 to murine T and natural killer cell functions. J Immunol. 1990; 145(11):3628-3634. (Immunogen: Blocking, Inhibition). View Reference
  10. Papavasiliou F, Misulovin Z, Suh H, Nussenzweig MC. The role of Ig beta in precursor B cell transition and allelic exclusion. Science. 1995; 268(5209):408-411. (Biology). View Reference
  11. Rakasz E, Hagen M, Sandor M, Lynch RG. Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules. Int Immunol. 1997; 9(1):161-167. (Biology). View Reference
  12. Rodewald HR, Awad K, Moingeon P, et al. Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-1092. (Biology). View Reference
  13. Teh SJ, Killeen N, Tarakhovsky A, Littman DR, Teh HS. CD2 regulates the positive selection and function of antigen-specific CD4- CD8+ T cells. Blood. 1997; 89(4):1308-1318. (Biology). View Reference
  14. Yagita H, Asakawa J, Tansyo S, Nakamura T, Habu S, Okumura K. Expression and function of CD2 during murine thymocyte ontogeny. Eur J Immunol. 1989; 19(12):2211-2217. (Biology). View Reference
  15. Yagita H, Nakamura T, Asakawa J, et al. CD2 expression in murine B cell lineage. Int Immunol. 1989; 1(1):94-98. (Biology). View Reference
  16. Yagita H, Nakamura T, Karasuyama H, Okumura K. Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells. Proc Natl Acad Sci U S A. 1989; 86(2):645-649. (Immunogen: Flow cytometry). View Reference
View All (16) View Less
751141 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.