The L138 monoclonal antibody (also known as Leu-M7) specifically binds to a glycosylated 150 kDa type II integral membrane zinc-metalloprotease. The CD13 antigen is also known as aminopeptidase N, APN, ANPEP, and gp150. The CD13 antigen is expressed on granulocytes, monocytes, mast cells, and granulocyte/macrophage progenitor cells (CFU-GM), but not on lymphocytes, platelets, or erythrocytes. It is expressed on most acute myeloid leukemia (AML) cells and some chronic myeloid leukemia (CML) cells. The CD13 antigen is also expressed on epithelial cells of the kidney, small intestine, and respiratory tract, as well as in synaptic membranes in the central nervous system (CNS). The CD13 antigen is involved in the metabolism of many regulatory peptides. Clustering of the CD13 antigen by various forms of ligation promotes the adhesion between monocytes and endothelial cells. The CD13 antigen is the receptor for human coronavirus 229E, the causative agent for some cases of upper respiratory infection.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).