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      Alexa Fluor™ 647 Mouse Anti-NTAL
      Alexa Fluor™ 647 Mouse Anti-NTAL
      Multicolor flow cytometric analysis of NTAL expression by Human peripheral blood leukocytes and Mouse splenic leukocytes.    Panel 1 - Human whole blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and then stained with BD Horizon™ BUV395 Mouse Anti-Human CD3 (Cat. No. 563546; Left Plots) and with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 570232; Top Plots) or Alexa Fluor™ 647 Mouse Anti-NTAL antibody (Cat. No. 570202; Bottom Plots) at 0.5 μg/ test. The bivariate pseudocolor density plots showing the correlated expression of NTAL (or Ig Isotype control staining) versus CD3 (Left Plots), or side light-scatter (SSC-A) signals (Right Plots) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes (Left Plots) or intact leukocyte populations (Right Plots).    Panel 2 - BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The leukocytes were then fixed using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer and then stained with PE Hamster Anti-Mouse CD3e (Cat. No. 553063) and with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Top Plot) or Alexa Fluor™ 647 Mouse Anti-NTAL antibody (Bottom Plot) at 0.5 μg/ test. The bivariate pseudocolor density plots showing the correlated expression of NTAL (or Ig Isotype control staining) versus CD3e were derived from gated events with the forward and side light-scatter characteristics of intact splenic lymphocytes.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor™ 647 Mouse Anti-NTAL
      Multicolor flow cytometric analysis of NTAL expression by Human peripheral blood leukocytes and Mouse splenic leukocytes.    Panel 1 - Human whole blood was treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to remove erythrocytes and fix leukocytes. The fixed leukocytes were permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and then stained with BD Horizon™ BUV395 Mouse Anti-Human CD3 (Cat. No. 563546; Left Plots) and with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 570232; Top Plots) or Alexa Fluor™ 647 Mouse Anti-NTAL antibody (Cat. No. 570202; Bottom Plots) at 0.5 μg/ test. The bivariate pseudocolor density plots showing the correlated expression of NTAL (or Ig Isotype control staining) versus CD3 (Left Plots), or side light-scatter (SSC-A) signals (Right Plots) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes (Left Plots) or intact leukocyte populations (Right Plots).    Panel 2 - BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The leukocytes were then fixed using BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer and then stained with PE Hamster Anti-Mouse CD3e (Cat. No. 553063) and with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Top Plot) or Alexa Fluor™ 647 Mouse Anti-NTAL antibody (Bottom Plot) at 0.5 μg/ test. The bivariate pseudocolor density plots showing the correlated expression of NTAL (or Ig Isotype control staining) versus CD3e were derived from gated events with the forward and side light-scatter characteristics of intact splenic lymphocytes.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      LAB; LAT2; linker for activation of B-cells; linker for activation of T-cells family member 2; non-T-cell activation linker
      Human (QC Testing), Mouse (Tested in Development)
      Mouse IgG1, κ
      Recombinant Cytoplasmic Domain (aa 91-243) of Human NTAL
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      7462
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      11. For U.S. patents that may apply, see bd.com/patents.
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      570202 Rev. 2
      Antibody Details
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      NAP-07

      The NAP-07 monoclonal antibody specifically recognizes NTAL (Non-T cell activation linker) which is also known as LAT2 (Linker for activation of T cells 2), LAB (Linker for activation of B cells) or Linker for activation of T-cells family member 2. NTAL is a ~30 kDa single-pass type III raft-localized transmembrane adaptor protein encoded by the LAT2 gene. NTAL is mainly expressed in B cells, natural killer cells, dendritic cells, monocytes, and mast cells but is absent in resting T cells. As an adaptor/scaffolding molecule, NTAL uses its tyrosine-based activation motifs to recruit signaling molecules such as Grb2 (Growth factor receptor-bound protein-2) into the immunoreceptor-signaling complex. This key adaptor protein is reportedly involved in the fine-tuning of lymphocyte activation and regulation of mast cell physiology.

      570202 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      570202 Rev.2
      Citations & References
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      View product citations for antibody "570202" on CiteAb

      Development References (6)

      1. Brdicka T, Imrich M, Angelisová P, et al. Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling.. J Exp Med. 2002; 196(12):1617-26. (Immunogen: Flow cytometry, Immunocytochemistry, Immunofluorescence, Western blot). View Reference
      2. Chiesa S, Mingueneau M, Fuseri N, et al. Multiplicity and plasticity of natural killer cell signaling pathways.. Blood. 2006; 107(6):2364-72. (Clone-specific: Intracellular Staining/Flow Cytometry, Western blot). View Reference
      3. Horejsí V, Zhang W, Schraven B. Transmembrane adaptor proteins: organizers of immunoreceptor signalling.. Nat Rev Immunol. 2004; 4(8):603-16. (Biology). View Reference
      4. Tůmová M, Koffer A, Simíček M, Dráberová L, Dráber P. The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho.. Eur J Immunol. 2010; 40(11):3235-45. (Biology). View Reference
      5. Volná P, Lebduska P, Dráberová L, et al. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL.. J Exp Med. 2004; 200(8):1001-13. (Clone-specific: Flow cytometry, Western blot). View Reference
      6. Yamasaki S, Ishikawa E, Sakuma M, et al. LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival.. Mol Cell Biol. 2007; 27(12):4406-15. (Biology). View Reference
      View All (6) View Less
      570202 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.