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      Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7

      BD Pharmingen™ Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7

      Clone F2.67 (also known as F4.67) (RUO)

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      Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7
      Flow cytometric analysis of TCR Vγ7 expression on viable Mouse splenic T cells.  BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with APC-Cy7 Hamster Anti-Mouse CD3e (Cat. No. 557596) and BD Horizon™ BV421 Hamster Anti-Mouse γδ T-Cell Receptor (Cat. No. 562892) antibodies and with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7 antibody (Cat. No. 569448/569449; Right Plot) at 1 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ7 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7
      Flow cytometric analysis of TCR Vγ7 expression on viable Mouse splenic T cells.  BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with APC-Cy7 Hamster Anti-Mouse CD3e (Cat. No. 557596) and BD Horizon™ BV421 Hamster Anti-Mouse γδ T-Cell Receptor (Cat. No. 562892) antibodies and with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat. No. 565357; Left Plot) or Alexa Fluor™ 647 Mouse Anti-Mouse TCR Vγ7 antibody (Cat. No. 569448/569449; Right Plot) at 1 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ7 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      T Cell Receptor Vγ7; TCR Vg7; TCR Vgamma7; Tcrg-V7; Trgv7
      Mouse (QC Testing)
      Mouse IgG2a, κ
      Mouse γδ T Cell Hybridoma Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      21641
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. For U.S. patents that may apply, see bd.com/patents.
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      569449 Rev. 2
      Antibody Details
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      F2.67

      The F2.67 monoclonal antibody specifically recognizes the variable gamma 7 region of the γ subunit of the mouse γδ T cell receptor for antigen, TCR Vγ7 (using the Heilig and Tonegawa nomenclature for mouse TCR γ and δ chains). TCR Vγ7 is encoded by the Trgv7 (T cell receptor gamma, variable 7) gene element. TCR Vγ7 is expressed by a subset of TCR γδ+ thymocytes in the late fetal and adult thymus and by γδ T cells in peripheral lymphoid tissues. TCR Vγ7+ γδ T cells predominate in intestinal epithelial tissue which contains a large proportion of these γδ T cells derived from extrathymic generation. Proteins encoded by Btnl1 (butyrophilin-like 1) and Btnl6 (butyrophilin-like 6) are expressed by intestinal epithelial cells. These butyrophilin-like molecules can reportedly shape the TCR-dependent development and function of TCR Vg7+ γδ T cells within the gut. TCR Vγ7+ γδ T cells help maintain the integrity of the intestinal mucosa guarding against cellular stress or damage caused by inflammation, transformation, or infection. The F2.67 antibody is useful for TCR Vγ7+ thymocyte and γδ T cell separations and analyzing TCR Vγ repertoires expressed by thymocytes, peripheral T cells, and T cell hybridomas in developmental and other experimental model systems.

      569449 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      569449 Rev.2
      Citations & References
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      View product citations for antibody "569449" on CiteAb

      Development References (10)

      1. Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).. Eur J Immunol. 2019; 49(10):1457-1973. (Clone-specific: Flow cytometry). View Reference
      2. Dalton JE, Cruickshank SM, Egan CE, et al. Intraepithelial gammadelta+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection.. Gastroenterology. 2006; 131(3):818-29. (Clone-specific: Flow cytometry). View Reference
      3. Garman RD, Doherty PJ, Raulet DH. Diversity, rearrangement, and expression of murine T cell gamma genes.. Cell. 1986; 45(5):733-42. (Biology). View Reference
      4. Heilig JS, Tonegawa S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes.. Nature. 322(6082):836-40. (Biology: Flow cytometry). View Reference
      5. Kashani E, Föhse L, Raha S, et al. A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.. Nat Commun. 2015; 6:6477. (Clone-specific: Flow cytometry). View Reference
      6. Monin L, Ushakov DS, Arnesen H, et al. γδ T cells compose a developmentally regulated intrauterine population and protect against vaginal candidiasis.. Mucosal Immunol. 2020; 13(6):969-981. (Clone-specific: Flow cytometry). View Reference
      7. Pereira P, Boucontet L. Rates of recombination and chain pair biases greatly influence the primary gammadelta TCR repertoire in the thymus of adult mice.. J Immunol. 2004; 173(5):3261-70. (Clone-specific: Flow cytometry). View Reference
      8. Pereira P, Hermitte V, Lembezat MP, Boucontet L, Azuara V, Grigoriadou K. Developmentally regulated and lineage-specific rearrangement of T cell receptor Valpha/delta gene segments.. Eur J Immunol. 2000; 30(7):1988-97. (Immunogen: Flow cytometry). View Reference
      9. Sell S, Dietz M, Schneider A, Holtappels R, Mach M, Winkler TH. Control of murine cytomegalovirus infection by γδ T cells.. PLoS Pathog. 2015; 11(2):e1004481. (Clone-specific: Flow cytometry). View Reference
      10. Zeng W, O'Brien RL, Born WK, Huang Y. Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity.. Methods Mol Biol. 2018; 1799:135-151. (Clone-specific: Flow cytometry). View Reference
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      569449 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.