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FITC Mouse Anti-Human IL-6
Product Details
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BD FastImmune™
Human
Mouse IgG1
Recombinant human IL-6 receptor antagonist
Intracellular staining (flow cytometry)
1.5 μg/mL
20 μL
3569
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340526 Rev. 1
Antibody Details
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AS12

The Anti-Hu–IL-6 antibody, clone AS12, is derived from the fusion of P3X63.Ag8.653 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-6.

The Anti-Human Interleukin-6 (Anti-Hu–IL-6) antibody recognizes a 21- to 29-kilodalton (kDa) polypeptide.

340526 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
340526 Rev.1
Citations & References
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Development References (24)

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  4. Breen EC, Rezai AR, Nakajuma K, et al. Infection with HIV is associated with elevated IL-6 levels and production. J Immunol. 1990; 144:480-484. (Biology).
  5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
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  7. Dokter WHA, Koopmans SB, Vellenga E. Effects of IL-10 and IL-4 on LPS-induced transcription factors (AP-1, NF-IL6 and NF-κB) which are involved in IL-6 regulation. Leukemia. 1996; 10:1308-1316. (Biology).
  8. Feldmann M, Brennan FM, Maini RN. Rheumatoid arthritis. Cell. 1996; 85:307-310. (Biology).
  9. Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Ann Rev Immunol. 1996; 14:397-440. (Biology).
  10. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H. Interferon β 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc Natl Acad Sci USA. 1987; 84:7251-7256. (Biology).
  11. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990; 63(6):1149-1157. (Biology). View Reference
  12. Iwakura Y, Tosu M, Yoshida E, et al. Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-1. Science. 1991; 253:1026-1028. (Biology).
  13. Kishimoto T, Akira S, Taga T. Interleukin-6 and its receptor: a paradigm for cytokines. Science. 1992; 258(5082):593-597. (Biology). View Reference
  14. Kishimoto T. The biology of interleukin-6. Blood. 1989; 74:43840. (Biology).
  15. Kohase M, May LT, Tamm I, Vilcek J, Sehgal PB. A cytokine network in human diploid fibroblasts: Interactions of β -interferons, tumor necrosis factor, platelet-derived growth factor, and interleukin-1. Mol Cell Biol. 1987; 7:273-280. (Biology).
  16. Koike K, Nakahata T, Takagi M, et al. Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture. J Exp Med. 1988; 168:879-890. (Biology).
  17. Libermann TA, Baltimore D. Activation of interleukin-6 gene expression through the NF-κB transcription factor. Mol Cell Biol. 1990; 10:2327-2334. (Biology).
  18. Lotz M, Jirik F, Kabouridis P, et al. B cell stimulating factor 2/interleukin 6 is a costimulant for human thymocytes and T lymphocytes. J Exp Med. 1988; 167:1253-1258. (Biology).
  19. May LT, Ghrayeb J, Santhanam U, et al. Synthesis and secretion of multiple forms of β2-interferon/B-cell differentiation factor 2/hepatocyte-stimulating factor by human fibroblasts and monocytes. J Biol Chem. 1988; 263:7760-7765. (Biology).
  20. Muraguchi A, Hirano T, Tang B, et al. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med. 1988; 167:332-344. (Biology).
  21. Muraguchi A, Kishimoto T, Miki Y, et al. T-cell replacing factor–(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J Immunol. 1981; 127:412-416. (Biology).
  22. Van Damme J, Opdenakker G, Simpson RJ, et al. Identification of the human 26-kD protein, interferon β 2 (IFN- β2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med. 1987; 165:914-919. (Biology).
  23. Van Snick, J. Interleukin 6: an overview. Ann Rev Immunol. 1990; 8:253-278. (Biology).
  24. Zhang Y, Broser M, Rom WN. Activation of the interleukin 6 gene by mycobacterium tuberculosis or lipopolysaccharide is mediated by nuclear factors NF-IL6 and NF-κB. Proc Natl Acad Sci USA. 1994; 91:2225-2229. (Biology).
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340526 Rev. 1

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