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V450 Rat Anti-Mouse Ly-6G
V450 Rat Anti-Mouse Ly-6G

Analysis of Ly-6G on mouse bone marrow. Bone marrow cells from C57BL/6 mice were stained either with a BD Horizon™ V450 Rat IgG2a, κ isotype control (Cat. No. 560377; unshaded histogram) or with the BD Horizon™ V450 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560603; shaded histogram).  Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Analysis of Ly-6G on mouse bone marrow. Bone marrow cells from C57BL/6 mice were stained either with a BD Horizon™ V450 Rat IgG2a, κ isotype control (Cat. No. 560377; unshaded histogram) or with the BD Horizon™ V450 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560603; shaded histogram).  Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Product Details
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BD Horizon™
Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Ly-6G-transfected EL4J Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_1727564
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560603 Rev. 2
Antibody Details
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1A8

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

560603 Rev. 2
Format Details
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V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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V450
Violet 405 nm
405 nm
450 nm
560603 Rev.2
Citations & References
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Development References (1)

  1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen). View Reference
560603 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.