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      Purified NA/LE Rat Anti-Mouse LPAM-1
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      This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [553153] or for additional support, contact your local applications specialist. Contact Us #
      Product Details
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      BD Pharmingen™
      Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2a, κ
      AKR/Cum mouse T lymphoma line TK1
      Flow cytometry (Routinely Tested), Blocking, Induction (Reported), Immunohistochemistry (Not Recommended)
      1.0 mg/ml
      AB_395065
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Recommended Assay Procedures

      Flow cytometry: Because expression of LPAM-1 on peripheral lymphocytes is at very low density, we recommend amplification of the signal through the use of a biotinylated second-step antibody plus a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061), for detection of the antigen by flow cytometry. Other reported applications include in vitro blocking of adhesion, induction of cellular aggregation, and in vivo blocking of lymphocyte homing to mucosal sites.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      553809 Rev. 11
      Antibody Details
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      DATK32

      The DATK32 monoclonal antibody specifically binds to an epitope specific for the α4β7 integrin heterodimer.  α4β7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells.  It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1.  DATK32  antibody induces α4β7-dependent lymphocyte aggregation, but it inhibits other α4β7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).

      553809 Rev. 11
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      553809 Rev.11
      Citations & References
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      Development References (5)

      1. Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Induction). View Reference
      2. Andrew DP, Rott LS, Kilshaw PJ, Butcher EC. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes. Eur J Immunol. 1996; 26(4):897-905. (Clone-specific: Blocking). View Reference
      3. Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Clone-specific: Blocking). View Reference
      4. Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994; 152(7):3282-3293. (Clone-specific: Blocking). View Reference
      5. Kilshaw PJ, Murant SJ. Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur J Immunol. 1991; 21(10):2591-2597. (Biology). View Reference
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      553809 Rev. 11

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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