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Purified Mouse Anti-Rat OX40 Ligand (CD252)
Product Details
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BD Pharmingen™
Tnfsf4; OX40 ligand; OX40L; TXGP1; Txgp1l; gp34; Ath-1; Ath1; CD134L
Rat (QC Testing)
Mouse BALB/c IgG1, κ
ACI-S1 rat T-cell lymphoma cell line
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_397393
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

We recommend, as a second-step, the biotinylated goat anti-mouse Ig polyclonal antibody (Cat. no. 553999), followed by Streptavidin-PE (Cat. no. 554061).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559957 Rev. 7
Antibody Details
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ATM-2

The ATM-2 antibody reacts with rat OX-40 Ligand (OX-40L, CD252), a member of the TNF Ligand superfamily.  Although there is controversy regarding mouse OX-40L expression, OX-40L has been shown to be expressed on human dendritic cells  and mouse activated B lymphocytes.  Rat OX-40L interacts with OX-40 Antigen (CD134) found predominantly on activated T cells. The OX-40L-OX-40 interaction is reciprocally costimulatory in that both T cells and B cells are activated in cross-linking. Rat OX-40L mRNA has been detected in various HTLV-I-transformed T-cell lines: F344-S1, WKA-S1, and ACI-S1.  In addition, total rat spleen cells activated with 5 ng/ml PMA and 0.5 µg/ml ionomycin for two days showed OX-40L expression, as determined by flow cytometric analysis, with the ATM-2 mAb.  As observed in the mouse and human systems, rat OX-40L also costimulates IL-2 production and proliferation of anti- D3-activated rat T cells.  The ATM-2 mAb blocks the costimulatory activity of OX-40L on rat T- cell proliferation.

559957 Rev. 7
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559957 Rev.7
Citations & References
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Development References (3)

  1. Calderhead DM, Buhlmann JE, van den Eertwegh AJ, Claassen E, Noelle RJ, Fell HP. Cloning of mouse Ox40: a T cell activation marker that may mediate T-B cell interactions. J Immunol. 1993; 151(10):5261-5271. (Biology). View Reference
  2. Ohshima Y, Tanaka Y, Tozawa H, Takahashi Y, Maliszewski C, Delespesse G. Expression and function of OX40 ligand on human dendritic cells. J Immunol. 1997; 159(8):3838-3848. (Immunogen: Blocking). View Reference
  3. Paterson DJ, Jefferies WA, Green JR. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987; 24(12):1281-1290. (Biology). View Reference
559957 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.