Search functionality is temporarily unavailable due to a system issue. 5:30 p.m - 10:30 p.m CEST on Sunday, January 18, 2026. Read More

If you encounter problems while ordering, please contact us at BDBCustomerService@bd.com

Purified Mouse Anti-Human CD3

BD Pharmingen™ Purified Mouse Anti-Human CD3

Name: Purified Mouse Anti-Human CD3
Brand: Purified Mouse Anti-Human CD3
SKU: 555337

Clone HIT3a (RUO)

Profile of peripheral blood lymphocytes analyzed on a BD FACScan™ (BDIS, San Jose, CA)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

Workshop Number: V 5T-CD03.05

RRID: AB_395743

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

Purified Mouse IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 555571

555337 Rev. 2

Antibody Details

HIT3a

The HIT3a monoclonal antibody specifically binds to the human CD3ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes. The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor. HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat. No. 555330/550368).

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555337 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

555337 Rev.2

Citations & References

View product citations for antibody "555337" on CiteAb

Development References (4)

Beverley PC, Callard RE. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981; 11(4):329-334. (Biology). View Reference
Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

View All (4)

555337 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.