Purified Mouse Anti-Human C1qRp (CD93)

This is a suggested alternate SKU for 551087. Contact us for additional support.

BD Pharmingen™ Purified Mouse Anti-Human C1qRp (CD93)

Name: Purified Mouse Anti-Human C1qRp (CD93)
Brand: BD Pharmingen™
SKU: 551454

Clone R3

(RUO)

Expression of C1qRP (CD93) by unstimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stained with Purified Mouse Anti-Human C1qRp (CD93) (0.125 µg, Cat. No. 551454, shaded histogram) or Purified Mouse IgM, κ Isotype Control (Cat. No.555581, open histogram) by using BD Biosciences Pharmingen's staining protocol. Secondary staining was performed with Biotin Goat Anti-Mouse Ig (Cat. No. 554061) and Streptavidin PE (Cat. No. 554061). Fluoresence histograms were derived from gated events with the forward and side light-scatter characteristics of viable monocytes.

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Product Details

Brand: BD Pharmingen™

Alternative Name: C1qRp

Reactivity: Human (QC Testing)

Isotype: Mouse IgM, κ

Immunogen: C1q-binding protein

Application: Flow cytometry (Routinely Tested), Immunoprecipitation, Western blot (Reported)

Concentration: 0.5 mg/ml

RRID: AB_394201

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunofluorescent Staining and Flow Cytometric Analysis: The staining technique and blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgM, κ isotype control (Cat. No. 555581) for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (eg, ≤ 0.5 µg Ab/1 million cells) (see Figure). For specific methodology, please visit the protocols section under "Multicolor Flow Cytometry" at http://www.bdbiosciences.com/us/s/resources.

Western Blotting and immunoprecipitation: When run under non-reducing conditions, Human CD93 migrates as a 100 kDa protein; due to high level of glycosylation CD93 migrates as 126 kDa under reducing conditions. The R3 antibody is suitable to detect CD93 by Western blotting and immunprecipitation as described; however, the antibody is not tested for this application at BD Biosciences Pharmingen. Reactivity of the antibody with the reduced protein is dramatically decreased.

Modulation of monocyte phagocytic activity: Pretreatment of monocytes with R3 neutralizes CD93-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics CD93- MBL- or SPA-mediated enhancement of phagocytosis; however, the antibody is not tested for this application at BD Biosciences Pharmingen.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Purified Mouse IgM, κ Isotype Control RUO

Size 0.1 mg Cat No. 555581

Biotin Goat Anti-Mouse Ig (Multiple Adsorption) RUO

Size 0.25 mg Cat No. 550337

PE Streptavidin RUO

Size 0.5 mg Cat No. 554061

551454 Rev. 2

Antibody Details

The immunogen used to raise R3 was C1q-binding protein preparation as described. Human C1qRp is a 631 a.a. protein (~66.5 kD) protein that is highly expressed on monocytes/macrophages, neutrophil granulocytes but not on T and B lymphocytes. C1qRp binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R3 is suitable to detect C1qRp expression on cells of myeloid lineage by flow cytometry, C1qRp in cellular lysates by Western blotting or immunoprecipitation. Pretreatment of monocytes with R3 neutralizes C1q-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics C1q- MBL- or SPA-mediated enhancement of phagocytosis as reported.

CDw93 (C1qRp) has been reported to define a human stem cell population with hematopoietic and hepatic potential.

551454 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

551454 Rev.2

Citations & References

View product citations for antibody "551454" on CiteAb

Development References (7)

Danet GH, Luongo JL, Butler G, et al. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential.. Proc Natl Acad Sci USA. 2002; 99(16):10441-5. (Biology). View Reference
Guan E, Robinson SL, Goodman EB, Tenner AJ. Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis. J Immunol. 1994; 152(8):4005-4016. (Biology). View Reference
Guan EN, Burgess WH, Robinson SL, Goodman EB, McTigue KJ, Tenner AJ. Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis. J Biol Chem. 1991; 266(30):20345-20355. (Biology). View Reference
Nepomuceno RR, Henschen-Edman AH, Burgess WH, Tenner AJ. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. Immunity. 1997; 6(2):119-129. (Biology). View Reference
Nepomuceno RR, Ruiz S, Park M, Tenner AJ. C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity. J Immunol. 1999; 162(6):3583-3589. (Biology). View Reference
Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998; 160(4):1929-1935. (Biology). View Reference
Tenner AJ. C1q receptors: regulating specific functions of phagocytic cells. Immunobiology. 1998; 199(2):250-264. (Biology). View Reference

View All (7)

551454 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.