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PE Mouse anti-Human IL-17A
PE Mouse anti-Human IL-17A

Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop™ (Cat. No. 554724) for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ reagents (Cat. No. 554714) followed by staining with PE anti-human IL-17A, PerCP anti-human CD4 (Cat. No. 347324), and either APC anti-human CD45RO (Cat. No. 559865; Left Panel) or Alexa Fluor® 647 anti-human IFN-γ (Cat. No. 557729; Middle Panel) or APC anti-human IL-4 (Cat. No. 554486; Right Panel). The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur™ System.

Flow cytometric analysis of PE anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop™ (Cat. No. 554724) for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ reagents (Cat. No. 554714) followed by staining with PE anti-human IL-17A, PerCP anti-human CD4 (Cat. No. 347324), and either APC anti-human CD45RO (Cat. No. 559865; Left Panel) or Alexa Fluor® 647 anti-human IFN-γ (Cat. No. 557729; Middle Panel) or APC anti-human IL-4 (Cat. No. 554486; Right Panel). The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur™ System.

Product Details
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BD Pharmingen™
IL-17; IL-17A; CTLA8; Cytotoxic T-lymphocyte-associated serine esterase 8
Human (QC Testing), Human (Reported)
Mouse IgG1, κ
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645514
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560436 Rev. 1
Antibody Details
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SCPL1362

Human IL-17A, also known as IL-17, is a proinflammatory cytokine that is encoded by the IL17A gene in chromosome 6. IL-17A is produced as a disulfide-linked homodimer comprised of two mature 136-amino acid polypeptides. It is a member of the IL-17 family of structurally related cytokines, designated IL-17A through IL-17F. Activated memory T cells, especially Th17 cells (specialized IL-17A-producing CD4+ T cells distinct from Th1 and Th2 cells) produce IL-17 and provide protective immunity against pathogens. Activated CD8+ T cells, γδT cells, NK cells and neutrophils can also be activated to produce IL-17A. IL-17A binds to and exerts its biological activity through IL-17 receptors (IL-17R) that are expressed by a variety of target cells including fibroblasts, epithelial and endothelial cells, monocytes/macrophages and mast cells. The ubiquitous IL-17R expression pattern may explain the broad tissue responsiveness to IL-17. IL-17 induces stromal cells to secrete cytokines and chemokines involved in inflammatory and hematopoietic processes. For example, IL-17 induces fibroblasts to produce IL-6, IL-8, G-CSF and express increased surface ICAM-1.  The SCPL1362 antibody reacts with human IL-17A.

560436 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560436 Rev.1
Citations & References
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Development References (6)

  1. Fossiez F, Djossou O, Chomarat P, et al. T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines. J Exp Med. 1996; 183(6):2593-2603. (Biology). View Reference
  2. Korn T, Oukka M, Kuchroo V, Bettelli E. Th17 cells: effector T cells with inflammatory properties. Semin Immunol. 2007; 19(6):362-371. (Biology). View Reference
  3. Moseley TA, Haudenschild DR, Rose L, Reddi AH. Interleukin-17 family and IL-17 receptors. Cytokine Growth Factor Rev. 2003; 14(2):155-174. (Biology). View Reference
  4. Weaver CT, Hatton RD, Mangan PR, Harrington LE. IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu Rev Immunol. 2007; 25:821-852. (Biology). View Reference
  5. Yao Z, Painter SL, Fanslow WC, et al. Human IL-17: a novel cytokine derived from T cells. J Immunol. 1995; 155(12):5483-5486. (Immunogen). View Reference
  6. Yao Z, Spriggs MK, Derry JM, et al. Molecular characterization of the human interleukin (IL)-17 receptor. Cytokine. 1997; 9(11):794-800. (Biology). View Reference
View All (6) View Less
560436 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.