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PE Mouse Anti-Human IL-13RA2 (CD213a2)

BD Pharmingen™ PE Mouse Anti-Human IL-13RA2 (CD213a2)

Clone 47 (also known as Clone 47; mAb47; 47 mAb)

(RUO)
PE Mouse Anti-Human IL-13RA2 (CD213a2)
Flow cytometric analysis of IL-13RA2 (CD213a2) expression on A-375 cells. Cells from the human A-375 (Malignant melanoma, ATCC CRL-1619IG-2) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human IL-13RA2 (CD213a2) antibody (Cat. No. 569002/569003; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing IL-13RA2 (CD213a2) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) A-375 cells. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of IL-13RA2 (CD213a2) expression on A-375 cells. Cells from the human A-375 (Malignant melanoma, ATCC CRL-1619IG-2) cell line were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human IL-13RA2 (CD213a2) antibody (Cat. No. 569002/569003; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing IL-13RA2 (CD213a2) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) A-375 cells. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
CD213A2; IL-13 receptor subunit alpha-2; IL-13R; IL-13Rα2; cancer/testis antigen 19
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human rhIL13Ra2hFc
Flow cytometry (Routinely Tested)
5 µl/test
3598
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Antibody Details
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47

Clone 47 is a monoclonal antibody that specifically recognizes the Human Interleukin-13 Receptor Subunit Alpha-2 (IL-13RA2), also known as IL-13Rα2 or CD213a2. IL-13RA2 (CD213a2) is a ~42 kDa type I transmembrane glycoprotein that is encoded by IL13RA2 which belongs to the type I cytokine receptor family. The extracellular domain is comprised of three extracellular fibronectin type III domains and a WSXWS motif that is followed by a transmembrane segment and a short cytoplasmic tail. Although this receptor primarily exists in a membrane-bound form, a soluble form may be generated upon enzymatic cleavage. IL-13RA2 (CD213a2) binds IL-13 with higher affinity than the moderate affinity IL-13RA1 (CD213a) subunit of the Type II IL-4R complex. This heterodimeric receptor complex is comprised of IL-4RA (CD124) and IL-13RA1 (CD213a) and transduces signals in response to either bound IL-4 or IL-13 ligands. IL-13RA2 (CD213a2) thus appears to act as a decoy receptor due to its short cytoplasmatic tail and lack of signaling motifs and thus controls the bioavailability and activity of IL-13. It is expressed by a subset of peripheral blood lymphocytes and immature dendritic cells. It is also known as cancer/testis antigen 19 (CT19) and may be highly expressed by a variety of malignant cells including high-grade gliomas.

Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
Citations & References
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View product citations for antibody "569003" on CiteAb

Development References (8)

  1. Balyasnikova IV, Wainwright DA, Solomaha E, et al. Characterization and immunotherapeutic implications for a novel antibody targeting interleukin (IL)-13 receptor α2.. J Biol Chem. 2012; 287(36):30215-27. (Immunogen). View Reference
  2. Hershey GK. IL-13 receptors and signaling pathways: an evolving web.. J Allergy Clin Immunol. 2003; 111(4):677-90; quiz 691. (Biology). View Reference
  3. Lupardus PJ, Birnbaum ME, Garcia KC. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2.. Structure. 2010; 18(3):332-42. (Biology). View Reference
  4. Penke LR, Ouchi H, Speth JM, et al. Transcriptional regulation of the IL-13Rα2 gene in human lung fibroblasts.. Sci Rep. 2020; 10(1):1083. (Biology). View Reference
  5. Tabata Y, Khurana Hershey GK. IL-13 receptor isoforms: breaking through the complexity.. Curr Allergy Asthma Rep. 2007; 7(5):338-45. (Biology). View Reference
  6. Takenouchi M, Hirai S, Sakuragi N, Yagita H, Hamada H, Kato K. Epigenetic modulation enhances the therapeutic effect of anti-IL-13R(alpha)2 antibody in human mesothelioma xenografts.. Clin Cancer Res. 2011; 17(9):2819-29. (Biology: Gel shift). View Reference
  7. Thaci B, Brown CE, Binello E, Werbaneth K, Sampath P, Sengupta S. Significance of interleukin-13 receptor alpha 2-targeted glioblastoma therapy.. Neuro Oncol. 2014; 16(10):1304-12. (Biology). View Reference
  8. Zola H, Swart B, Nicholson I, Voss E. CD213α1 and 2. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:359-360.
View All (8) View Less

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