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PE Mouse anti-Human CD144
PE Mouse anti-Human CD144

Analysis of CD144 on Human Umbilical Vein Endothelial Cells (HUVECs).   HUVECs were stained with either PE Mouse Anti-Human CD144 (solid line) or PE Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 555749, dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Analysis of CD144 on Human Umbilical Vein Endothelial Cells (HUVECs).   HUVECs were stained with either PE Mouse Anti-Human CD144 (solid line) or PE Mouse IgG1, κ Isotype Control (clone MOPC-21, Cat. No. 555749, dotted line). Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Product Details
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BD Pharmingen™
VE-cadherin; Cadherin-5; CDH5; Vascular endothelial cadherin
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
VI E092
1003
AB_1645502
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560410 Rev. 3
Antibody Details
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55-7H1

The 55-7H1 antibody monoclonal antibody specifically binds to a calcium-independent epitope on Cadherin-5, a member of the cadherin family of calcium-dependent adhesion molecules. Cadherin-5 is also known as CD144 or VE-Cadherin. CD144 is expressed on endothelial cells in vivo and in vitro. It may play a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium.

560410 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560410 Rev.3
Citations & References
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Development References (3)

  1. Breier G, Breviario F, Caveda L, et al. Molecular cloning and expression of murine vascular endothelial-cadherin in early stage development of cardiovascular system.. Blood. 1996; 87(2):630-41. (Biology). View Reference
  2. Lampugnani MG, Resnati M, Raiteri M, et al. A novel endothelial-specific membrane protein is a marker of cell-cell contacts.. J Cell Biol. 1992; 118(6):1511-22. (Biology). View Reference
  3. Vincent PA, Xiao K, Buckley KM, Kowalczyk AP. VE-cadherin: adhesion at arm's length.. Am J Physiol, Cell Physiol. 2004; 286(5):C987-97. (Biology). View Reference
560410 Rev. 3

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.