The 218820 monoclonal antibody specifically recognizes CD369 which is also known as Dendritic cell-associated C-type lectin 1 (DC-associated C-type lectin 1, Dectin-1, or Dectin1), or Beta-glucan receptor (Bgr). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7 member a). CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). It is expressed on monocytes/macrophages, neutrophils, and some populations of dendritic cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 (Clec7a)-mediated signaling may play roles in leucocyte responses including phagocytosis or enhanced cytokine production.
The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 750-nm. BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass. Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.