The 218820 monoclonal antibody specifically recognizes CD369 which is also known as Dendritic cell-associated C-type lectin 1 (DC-associated C-type lectin 1, Dectin-1, or Dectin1), or Beta-glucan receptor (Bgr). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7 member a). CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain, a transmembrane segment, and a cytoplasmic tail with an immunoreceptor tyrosine-based activation motif (ITAM). It is expressed on monocytes/macrophages, neutrophils, and some populations of dendritic cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 (Clec7a)-mediated signaling may play roles in leucocyte responses including phagocytosis or enhanced cytokine production.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.