The CLB-IVC7 monoclonal antibody specifically binds to CD36 which is also known as Glycoprotein IIIb (GPIIIb, GP3B), Platelet glycoprotein IV (GPIV), Fatty acid translocase (FAT), Platelet collagen receptor (PASIV), Scavenger receptor class B member 3 (SCARB3) or Thrombospondin receptor. CD36 is a ~88 kDa transmembrane that belongs to the scavenger receptor superfamily. The CD36 antigen is expressed on platelets, megakaryocytes, monocytes, macrophages, dendritic cells, erythroid precursors, adipocytes, and some endothelial and epithelial cells. The CD36 antigen is the receptor for extracellular matrix proteins such as collagen and thrombospondin. The CD36 antigen can mediate the adhesion of erythrocytes infected with the human malaria parasite, Plasmodium falciparum. The CD36 antigen functions as a scavenger receptor in macrophages.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.