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APC Rat anti-Mouse CD229.1
APC Rat anti-Mouse CD229.1

Flow cytometric analysis of APC-conjugated anti-mouse CD229.1 on mouse splenocytes.

Splenocytes from BALB/c mice were stained with APC anti-mouse CD229.1 (clone 30C7, Cat. No. 560188)  and compared to splenocytes stained with an APC rat IgG2a isotype control (clone R35-95, Cat. No. 553932). The isotype control is represented by a dashed line and the APC anti-mouse CD229.1 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System.

Flow cytometric analysis of APC-conjugated anti-mouse CD229.1 on mouse splenocytes.

Splenocytes from BALB/c mice were stained with APC anti-mouse CD229.1 (clone 30C7, Cat. No. 560188)  and compared to splenocytes stained with an APC rat IgG2a isotype control (clone R35-95, Cat. No. 553932). The isotype control is represented by a dashed line and the APC anti-mouse CD229.1 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System.

Product Details
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BD Pharmingen™
Ly-9.1; T100; CD229; Lgp100; SLAMF3
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
SJL/J mouse splenocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_1645221
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560188 Rev. 1
Antibody Details
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30C7

The 30C7 monoclonal antibody specifically binds to CD229.1, the Ly-9.1 (Lgp-100a) surface glycoprotein alloantigen found on most thymocytes, peripheral T and B cells, bone marrow lymphoid cells, hematopoietic progenitors, and lymphomas of most strains (e.g., AKR, Balb/c, CBA, SJL).  30C7 mAb does not react with the Ly-9.2 alloantigen expressed on mice derived from the C57/J line (e.g., C57BL, C57BR, C57L, C58).

560188 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
560188 Rev.1
Citations & References
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Development References (3)

  1. Lanier LL, Warner NL, Ledbetter JA, Herzenberg LA. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J Immunol. 1981; 127(4):1691-1697. (Clone-specific). View Reference
  2. Ledbetter JA, Goding JW, Tsu TT, Herzenberg LA. A new mouse lymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibody. Immunogenetics. 1979; 8(1):347-360. (Immunogen).
  3. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979; 47:63-90. (Biology). View Reference
560188 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.