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Alexa Fluor® 647 Rat anti-Mouse RANKL/TRANCE
Alexa Fluor® 647 Rat anti-Mouse RANKL/TRANCE

Analysis of Alexa Fluor® 647 Rat anti-Mouse RANKL on mouse unstimulated and ConA-stimulated splenocytes. Unstimulated splenocytes (left panel) and ConA activated splenocytes (right panel) were stained with either Alexa Fluor® 647 Rat anti-Mouse RANKL (solid line) or Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (clone R35-95, Cat. No. 557906, dotted line). Flow cytometry was performed on a BD FACSCanto™ II System.

Analysis of Alexa Fluor® 647 Rat anti-Mouse RANKL on mouse unstimulated and ConA-stimulated splenocytes. Unstimulated splenocytes (left panel) and ConA activated splenocytes (right panel) were stained with either Alexa Fluor® 647 Rat anti-Mouse RANKL (solid line) or Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (clone R35-95, Cat. No. 557906, dotted line). Flow cytometry was performed on a BD FACSCanto™ II System.

Product Details
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BD Pharmingen™
TRANCE; TRANCEODF; OPGL; sOdf; CD254; OPTB2; hRANKL2
Mouse (QC Testing)
Rat IgG2a
Mouse recombinant RANKL
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_1645206
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
560296 Rev. 1
Antibody Details
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IK22-5

The IK22-5 monoclonal antibody specifically recognizes CD254, which is also known as, Receptor Activator of NF-kB Ligand (RANKL), TNF-Related Activation-Induced Cytokine Receptor (Trance), Osteoprotegerin Ligand (OPGL), or Osteoclast Differentiation Factor (ODF). CD254/RANKL is likewise known as Tnfsf11, a member of the tumor necrosis factor family. RANKL has been implicated in interactions between T cells and dendritic cells. RANKL binds to RANK expressed on dendritic cells and upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival.  RANKL is also important in T- and B-cell maturation. RANKL can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL-deficient mice show severe osteoporosis and complete absence of osteoclasts as a result of lack of osteogenesis.  It is expressed abundantly by mouse activated T cells, not B cells.  It is also expressed on osteoclasts.

560296 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
560296 Rev.1
Citations & References
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Development References (3)

  1. Ando T, Ichikawa J, Wako M et al. TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. Arthritis Res Ther. 2006; 8(5):R146. (Biology). View Reference
  2. Hofbauer LC, Schoppet M. Clinical implications of the osteoprotegerin/RANKL/RANK system for bone and vascular diseases. 2004; 292(4):490-495. (Biology). View Reference
  3. Kamijo S, Nakajima A, Ikeda K et al. Amelioration of bone loss in collagen-induced arthritis by neutralizing anti-RANKL monoclonal antibody. Biochem Biophys Res Commun. 2006; 347(1):124-132. (Clone-specific). View Reference
560296 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.