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Alexa Fluor® 647 Mouse anti-CDX-2
Alexa Fluor® 647 Mouse anti-CDX-2

Analysis of CDX-2 on human colorectal adenocarcinoma cell line DLD-1. DLD-1 cells (ATCC CCL-221) were fixed BD Cytofix™ (Cat. No. 554655) and permeabilized with the BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), then stained with Alexa Fluor® 647 Mouse anti-CDX-2 (solid line)  or Alexa Fluor® 647 Mouse IgG1 · Isotype Control (clone MOPC-21, Cat. No. 557732) (dashed line). This antibody also works in BD™ Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Alexa Fluor® 647 Mouse anti-CDX-2

Analysis of CDX-2 on spontaneously differentiated embryonic stem cells.  Human embryonic stem cells (H9; WiCell, Madison, WI) were spontaneously differentiated in a 6-well plate for 3 weeks.  Cells were fixed with BD Cytofix™ (Cat. No. 554655) and permeabilized with the BD™ Phosflow Perm/Wash

Buffer I (Cat. No. 557885), then stained with Alexa Fluor® 647 Mouse anti-CDX-2 and PE Mouse anti-Oct3/4 (clone 40/Oct-3, Cat. No. 560186).

Analysis of CDX-2 on human colorectal adenocarcinoma cell line DLD-1. DLD-1 cells (ATCC CCL-221) were fixed BD Cytofix™ (Cat. No. 554655) and permeabilized with the BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), then stained with Alexa Fluor® 647 Mouse anti-CDX-2 (solid line)  or Alexa Fluor® 647 Mouse IgG1 · Isotype Control (clone MOPC-21, Cat. No. 557732) (dashed line). This antibody also works in BD™ Phosflow Perm Buffer I and II. Flow cytometry was performed on a BD LSR™ II flow cytometry system.

Analysis of CDX-2 on spontaneously differentiated embryonic stem cells.  Human embryonic stem cells (H9; WiCell, Madison, WI) were spontaneously differentiated in a 6-well plate for 3 weeks.  Cells were fixed with BD Cytofix™ (Cat. No. 554655) and permeabilized with the BD™ Phosflow Perm/Wash

Buffer I (Cat. No. 557885), then stained with Alexa Fluor® 647 Mouse anti-CDX-2 and PE Mouse anti-Oct3/4 (clone 40/Oct-3, Cat. No. 560186).

Product Details
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BD Pharmingen™
CDX2, CDX3, CDX-3
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CDX-2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645405
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
560395 Rev. 1
Antibody Details
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M39-711

The M39-711 monoclonal antibody reacts with caudal-type homeobox transcription factor 2 (CDX-2).  In embryonic development, CDX-2 is first expressed in the outer layer of the morula and later in the trophoblast, but not in embryonic stem cells.  Along with Oct3/4, it is involved in the segregation of the trophoectoderm lineage from the inner cell mass.  In the adult, CDX-2 expression is limited to the mucosa of the small and large intestines, where it regulates the synthesis of several intestine-specific proteins.  Loss of CDX-2 expression is observed in some colorectal carcinomas, while gain of CDX-2 expression has been observed in gastric tumors and acute myeloid leukemias.

560395 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
560395 Rev.1
Citations & References
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Development References (6)

  1. Hinoi T, Loda M, Fearon ER. Silencing of CDX2 expression in colon cancer via a dominant repression pathway. J Biol Chem. 2003; 278(45):44608-44616. (Biology). View Reference
  2. Qualtrough D, Hinoi T, Fearon E, Paraskeva C. Expression of CDX2 in normal and neoplastic human colon tissue and during differentiation of an in vitro model system. Gut. 2002; 51:184-190. (Biology). View Reference
  3. Ralston A, Rossant J. Genetic regulation of stem cell origins in the mouse embryo. Clin Genet. 2005; 68(2):106-112. (Biology). View Reference
  4. Rawat VP, Cusan M, Deshpande A, et al. Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of (12;13)(p13;q12) acute myeloid leukemia. Proc Natl Acad Sci U S A. 2004; 101(3):817-822. (Biology). View Reference
  5. Scholl C, Bansal D, Döhner K, et al. The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis. J Clin Invest. 2007; 117(4):865-868. (Biology). View Reference
  6. Tsukamoto T, Mizoshita T, Tatematsu M. Gastric-and-intestinal mixed-type intestinal metaplasia: aberrant expression of transcription factors and stem cell intestinalization. Gastric Cancer. 2006; 9(3):156-166. (Biology). View Reference
View All (6) View Less
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Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.