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Purified Mouse Anti-Csk
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat Csk aa. 1-156
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
50 kDa
250 µg/ml
AB_397488
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610080 Rev. 7
Antibody Details
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52/Csk

The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60[c-src], p59[fyn], p53/56[lyn], p56[lck], and p62[yes]. This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59[fyn] and p53/56[lyn]. These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.

610080 Rev. 7
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610080 Rev.7
Citations & References
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Development References (5)

  1. Bergman M, Mustelin T, Oetken C. The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity. EMBO J. 1992; 11(8):2919-2924. (Biology). View Reference
  2. Bénistant C, Bourgaux JF, Chapuis H, Mottet N, Roche S, Bali JP. The COOH-terminal Src kinase Csk is a tumor antigen in human carcinoma. Cancer Res. 2001; 61(4):1415-1420. (Clone-specific: Immunofluorescence, Western blot). View Reference
  3. Okada M, Nada S, Yamanashi Y, Yamamoto T, Nakagawa H. CSK: a protein-tyrosine kinase involved in regulation of src family kinases. J Biol Chem. 1991; 266(36):24249-24252. (Biology). View Reference
  4. Takayama Y, Tanaka S, Nagai K, Okada M. Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999; 274(4):2291-2297. (Clone-specific: Immunoprecipitation). View Reference
  5. Wan Y, Bence K, Hata A, Kurosaki T, Veillette A, Huang XY. Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling. J Biol Chem. 1999; 272(27):17209-17215. (Clone-specific: In vitro kinase assay, Western blot). View Reference
View All (5) View Less
610080 Rev. 7

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.