Skip to main content Skip to navigation
Purified Mouse Anti- 14-3-3
Purified Mouse Anti- 14-3-3

Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody.

Purified Mouse Anti- 14-3-3

Immunofluorescence staining of SKN cells (Human neuroblastoma).

Western blot analysis of 14-3-3 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1: 1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Mouse Anti- 14-3-3 antibody.

Immunofluorescence staining of SKN cells (Human neuroblastoma).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog (Tested in Development)
Mouse IgM
Human 14-3-3
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
29 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561465 Rev. 1
Antibody Details
Down Arrow Up Arrow
12/14-3-3

The family of 14-3-3 proteins has been implicated in numerous cellular processes such as neurotransmission, phospholipase activity, and calcium signaling.  The 14-3-3 protein can associate with several proteins involved in cell proliferation, such as Raf-1, Bcr-Abl, middle T antigen, and CDC25 phosphatase.  It appears that 14-3-3 proteins enhance the kinase activity of the Raf proto-oncogene equally as well as Ras, thus, implicating 14-3-3 proteins in the Raf/Ras/Map kinase signaling pathway.  However, in spite of 14-3-3 direct association with the CDC25 phosphatase, this interaction does not increase the phosphatase activity.  It has been reported that this antibody detects 14-3-3 subunits β, γ, η, θ, and ζ , but not ε nor σ.

561465 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
561465 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Ayllon V, Cayla X, Garcia A, et al . Bcl-2 targets protein phosphatase 1 alpha to Bad. J Immunol. 2001; 166(12):7345-7352. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Conklin DS, Galaktionov K, Beach D.. 14-3-3 proteins associate with cdc25 phosphatases.. Proc Natl Acad Sci U S A. 1995; 92(17):7892-7896. (Biology). View Reference
  3. Freed E, Symons M, Macdonald SG, McCormick F, Ruggieri R.. Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation.. Science. 1994 ; 265(5179):1713-1716. (Biology: Western blot). View Reference
  4. Mamane Y, Grandvaux N, Hernandez E, et al. Repression of IRF-4 target genes in human T cell leukemia virus-1 infection. Nature. 2002; 21(44):6751-6765. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
  5. Ostrerova N, Petrucelli L, Farrer M, Mehta N,. alpha-Synuclein shares physical and functional homology with 14-3-3 proteins.. J Neurosci. 1999; 19(14):5782-5791. (Biology: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
561465 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.