ELISA Capture: Purified hIL4R-M10.1 anti-human IL-4R antibody (Cat. No. 552472) is useful as a capture antibody for a sandwich ELISA which measures human IL-4R protein levels. Purified hIL4R-M10.1 anti-human IL-4R antibody can be paired with biotinylated hIL4R-M.8.2.2 anti-human IL-4R (Cat. No. 552473) as the detection antibody, with recombinant soluble human IL-4R as the standard. This capture antibody should be titrated between 1 - 4 µg/ml to determine the optimal coating concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant human IL-4R ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see BD Pharmingen's Immune Function Handbook, at
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: IL-1RI, IL-1RII, IL-1α, IL-1ß, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, GM-CSF, TGFß, IFN γ, CD40L, TRAIL, FasL.