ELISA Detection: Biotinylated hIL4R-M8.2.2 (Cat. No. 552473) serves as the detection antibody in a sandwich ELISA for measuring human soluble IL-4R protein levels. Biotinylated hIL4R-M8.2.2 antibody can be paired with the purified hIL4R-M10.1 anti-human IL-4R (Cat No. 552472) using recombinant human soluble IL-4R as the standard. This detection antibody should be titrated between 0.5 - 1 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant soluble human IL-4R ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see BD Pharmingen's Immune Function Handbook, which is posted on our website at www.BDBiosciences.com.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: IL-1RI, IL-1RII, IL-1α, IL-1ß, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, GM-CSF, TGFß, IFN-ß, CD40L, TRAIL, FasL.