RY586 Mouse Anti-Biotin

BD OptiBuild™ RY586 Mouse Anti-Biotin

Name: RY586 Mouse Anti-Biotin
Brand: BD OptiBuild™
SKU: 758032

Clone BK-1/39

(RUO)

Product Details

Brand: BD OptiBuild™

Isotype: Mouse IgG1, κ

Immunogen: Biotin-conjugated Keyhole Limpet Hemocyanin (KLH)

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
CF™ is a trademark of Biotium, Inc.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

RY586 Mouse IgG1, κ Isotype Control RUO

Size 50 µg Cat No. 568097

758032 Rev. 1

Antibody Details

BK-1/39

The BK-1/39 monoclonal antibody specifically recognizes biotin. Biotin is a water-soluble B complex vitamin that functions as a coenzyme which is required for the cellular metabolism of proteins and fats and the production of fatty acids. Although required by all organisms, biotin synthesis is limited to bacteria, yeasts, molds, algae, and some plants. Biotin is also useful for tagging molecules such as nucleic acids or proteins including antibodies. The BK-1/39 antibody can used to detect biotinylated target molecules and antibodies especially when signal amplification is desired. Fluorescent BK-1/39 antibody conjugates can provide a useful alternative to fluorescent avidin conjugates in order to minimize background staining and maximize signal intensity.

758032 Rev. 1

Format Details

RY586

The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.

Format: RY586

Excitation Source: Yellow-Green 561 nm

Excitation Max: 564 nm

Emission Max: 586 nm

758032 Rev.1

Citations & References

View product citations for antibody "758032" on CiteAb

Development References (4)

Levit-Zerdoun E, Becker M, Pohlmeyer R, et al. Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.. J Immunol. 2016; 196(5):2348-60. (Clone-specific: Flow cytometry). View Reference
Mavrangelos C, Swart B, Nobbs S, Nicholson IC, Macardle PJ, Zola H. Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagents.. J Immunol Methods. 2004; 289(1-2):169-78. (Methodology: Flow cytometry). View Reference
Pishesha N, Bilate AM, Wibowo MC, et al. Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease.. Proc Natl Acad Sci U S A. 2017; 114(12):3157-3162. (Clone-specific: Flow cytometry). View Reference
Zempleni J, Wijeratne SS, Hassan YI. Biotin.. Biofactors. 2009; 35(1):36-46. (Biology). View Reference

View All (4)

758032 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.