R718 Mouse Anti-Human TCR Vβ5 Related

BD Horizon™ R718 Mouse Anti-Human TCR Vβ5 Related

Name: R718 Mouse Anti-Human TCR Vβ5 Related
Brand: R718 Mouse Anti-Human TCR Vβ5 Related
SKU: 570065

Clone MEM-262 (RUO)

Multiparameter flow cytometric analysis of TCR Vβ5 Related expression on Human peripheral blood T lymphocytes. Human peripheral blood was stained with BD Horizon™ BB515 Mouse Anti-Human CD3 antibody (Cat. No. 564465) and with either BD Horizon™ R718 Mouse IgG2a, κ Isotype Control (Cat. No. 566949; Left Plots) or BD Horizon™ R718 Mouse Anti-Human TCR Vβ5 Related antibody (Cat. No. 570065/570146; Right Plots). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plots showing the correlated expression of TCR Vβ5 Related (or Ig Isotype control staining) versus CD3 (Upper Plots) or side-light scatter (SSC-A) signals (Lower Plots) were derived from gated events with the forward and side light-scatter characteristics of intact human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Horizon™

Alternative Name: MEM-262 Antigen; TCR Vβ5.3 Related; TCR Vb5; TCR Vbeta5; TCRV beta 5

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Immunogen: TCR Vβ5.3+ Human HPB-ALL Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 6957

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

R718 Mouse IgG2a, κ Isotype Control RUO

Size 50 µg Cat No. 566949

BB515 Mouse Anti-Human CD3 RUO

Size 100 Tests Cat No. 564465

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

View Details

570065 Rev. 1

Antibody Details

MEM-262

The MEM-262 monoclonal antibody recognizes an epitope on the extracellular region of the beta chain of the human αβ T cell receptor for antigen (TCR) expressed by a TCR Vβ5.3+ human HPB-ALL cell line as well as a small subset of peripheral blood T cells. The peripheral T cell subset recognized by the MEM-262 antibody is larger than that recognized by other TCR Vβ5.3-specific antibodies and so the recognized epitope may be referred to as TCR Vβ5 Related. This antibody is useful for multiparameter analyses designed to study the nature of MEM-262-positive T cells including normal T cells and T cell clones or T cell lines. It is also useful for analyzing TCR Vβ repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models. This monoclonal antibody can reportedly stimulate the proliferation of MEM-262-positive T cells.

570065 Rev. 1

Format Details

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: R718

Excitation Source: Red 627-640 nm

Excitation Max: 695 nm

Emission Max: 718 nm

570065 Rev.1

Citations & References

View product citations for antibody "570065" on CiteAb

Development References (3)

Gertow J, Berglund S, Okas M, et al. Characterization of long-term mixed donor-donor chimerism after double cord blood transplantation.. Clin Exp Immunol. 2010; 162(1):146-55. (Clone-specific: Flow cytometry). View Reference
Giudice V, D'Addona M, Montuori N, Selleri C. The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.. Cancers (Basel). 2021; 13(18):4513. (Clone-specific: Flow cytometry). View Reference
Pavlistová D, Drbal K, Hilgert I, Horejsí V. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor.. Immunol Lett. 2003; 88(2):105-8. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Stimulation). View Reference

570065 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.