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R718 Mouse Anti-Human HLA-C
Product Details
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BD OptiBuild™
HLAC; HLC-C; HLA-JY3; MHC class I antigen C; PSORS1; HLA-E
Human (Tested in Development)
Mouse IgG2b, κ
Purified Human Tamarin MHC Class I Molecules
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Researchers should determine the optimal concentration of this reagent for their individual applications.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
751891 Rev. 1
Antibody Details
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DT-9

The DT-9 monoclonal antibody specifically recognizes Human Leukocyte Antigen (HLA-C), a polymorphic major histocompatibility complex (MHC) class I antigen. HLA-C is a heterodimer comprised of the HLA-C alpha chain, a ~45 kDa type I transmembrane glycoprotein, and a ~12 kDa Beta-2 (β2)-microglobulin light chain. HLA-C is expressed on nearly all cells, and plays a role in the antigen-specific, MHC-restricted presentation of small peptides to CD8+ T cells in the generation of immunity or tolerance. HLA-C may also bind to regulatory MHC class I antigen-selective receptors expressed by CD8+ T cells and natural killer (NK) cells.  The DT-9 antibody also recognizes some rare HLA-A and HLA-B allotypes and the nonclassical HLA-E molecule.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.

751891 Rev. 1
Format Details
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
751891 Rev.1
Citations & References
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Development References (6)

  1. Apps R, Qi Y, Carlson JM, et al. Influence of HLA-C expression level on HIV control.. Science. 2013; 340(6128):87-91. (Clone-specific: Flow cytometry). View Reference
  2. Braud VM, Allan DS, O'Callaghan CA, et al. HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C.. Nature. 1998; 391(6669):795-9. (Clone-specific: Flow cytometry). View Reference
  3. Braud VM, Allan DS, Wilson D, McMichael AJ. TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide.. Curr Biol. 1998; 8(1):1-10. (Immunogen: Flow cytometry, Immunoprecipitation). View Reference
  4. Corrah TW, Goonetilleke N, Kopycinski J, et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression.. J Virol. 2011; 85(7):3367-74. (Clone-specific: Flow cytometry). View Reference
  5. Thomas R, Apps R, Qi Y, et al. HLA-C cell surface expression and control of HIV/AIDS correlate with a variant upstream of HLA-C.. Nat Genet. 2009; 41(12):1290-4. (Clone-specific: Flow cytometry). View Reference
  6. Zipeto D, Beretta A. HLA-C and HIV-1: friends or foes?. Retrovirology. 2012; 9:39. (Biology). View Reference
View All (6) View Less
751891 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.